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Extracts of Dendropanax morbifera leaves have modulatory effects on neuroinflammation in microglia

Title
Extracts of Dendropanax morbifera leaves have modulatory effects on neuroinflammation in microglia
Translated Title
미세아교세포에서 에틸 아세테이트를 용매로 한 황칠나무 추출물의 신경염증 억제 효과
Authors
Park, Sin Woo
DGIST Authors
Park, Sin Woo; Yu, Seong Woon; Jang, Jae Eun
Advisor(s)
Yu, Seong Woon
Co-Advisor(s)
Jang, Jae Eun
Issue Date
2015
Degree Date
2015. 2
Type
Thesis
Keywords
Dendropanax morbiferaneuroinflammationmicrogliaMAPKNF-κB황칠나무신경 염증미세아교세포
Abstract
Dendropanax morbifera (D. morbifera) is an endemic species in Korea and distributes in the southern part of Korea. Its leaves, stems, roots and seeds have been used in folk medicine for various diseases. Recently some researchers suggest possibility of this tree as a medical application, until now the functional role and underlying regulatory mechanisms of this tree in neuroinflammation remain unclear. So, we focused on the modulatory effects of this tree on neuroinflammation. Here, we demonstrate that ethyl acetate extracts of D. morbifera leaves (DMLE) have modulatory effects on neuroinflammation in BV2 microglial cells. DMLE treatment suppressed production of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), nitric oxide (NO) and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated BV2 cells. DMLE treatment also attenuated phosphorylation of mitogen-activated protein kinases (MAPKs) and activation of nuclear factor-kappaB (NF-κB). Furthermore, DMLE suppressed expression of the genes related with pro-inflammatory M1 stage whereas alternative, anti-inflammatory M2 stage-related genes were up-regulated by DMLE. These results indicate that DMLE may have therapeutic effects for treatment of neurodegenerative diseases by suppressing neuroinflammation. Moreover, this research contribute to discover natural agent from Dendropanax morbifera and it could be an effective agent for the treating neurodegenerative diseases associated with neuroinflammation. ⓒ 2015 DGIST
Table Of Contents
I. Introduction 1 -- 1.1 Background information 1 -- II. Methods and Materials 4 -- 2.1. Chemicals and reagents 4 -- 2.2. Cell culture 4 -- 2.3. Cell viability 4 -- 2.4. Nitrite assay 5 -- 2.5. ELISA 5 -- 2.6. Western blot analysis 5 -- 2.7. RT-PCR 6 -- 2.8. NF-κB luciferase reporter assay 6 -- 2.9. Statistical analyses 7 -- III. Results 9 -- 3.1. DMLE suppresses production of pro-inflammatory cytokines 9 -- 3.2. DMLE blocks activation of MAPKs 10 -- 3.3. DMLE attenuates NF- κB activity 13 -- 3.4. DMLE induced expression of M2 stage related genes 21 -- IV. Discussion 25 -- V. References 29
URI
http://dgist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001923135
http://hdl.handle.net/20.500.11750/1383
DOI
10.22677/thesis.1923135
Degree
Master
Department
Brain Science
University
DGIST
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesThesesMaster


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