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Caffeine reduces LPS-induced pro-inflammatory microglial response and autophagic suppression by inducing autophagy.

Title
Caffeine reduces LPS-induced pro-inflammatory microglial response and autophagic suppression by inducing autophagy.
Authors
Kim, Eun Jung
DGIST Authors
Kim, Eun Jung; Yu, Seong Woon; Choi, Ji Woong
Advisor(s)
Yu, Seong Woon
Co-Advisor(s)
Choi, Ji Woong
Issue Date
2016
Available Date
2016-08-18
Degree Date
2016. 8
Type
Thesis
Keywords
AutophagyMicrogliaCaffeineNeuroinflammationLipopolysaccharide미세아교세포카페인뇌염증자가소화작용
Abstract
Background Microglia are the resident immune cells in the brain that are sensitive to changes in their local environment. They respond to pathogen or danger signals by triggering brain immune response. Many drugs working on the nervous system can target microglia as well as neurons. Caffeine is the most widely consumed psychoactive drug across various age groups worldwide. However, little is known whether caffeine can affect microglia function. This study is aimed to examine whether caffeine regulates microglia functions and autophagy is involved in the action of caffeine on microglia, since autophagy plays a critical role in cellular immune response. ⓒ 2016 DGIST
Table Of Contents
1. Introduction 1 -- 2. Materials and methods 4 -- 2.1 Antibodies and reagents 4 -- 2.2 Cell culture 4 -- 2.3 plasmids and magnetofection 5 -- 2.4 Sh-RNA Lentiviral production 5 -- 2.5 Western blotting 5 -- 2.6 Immunocytochemistry 6 -- 2.7 qRT-PCR 6 -- 2.8 Enzyme-linked immunosorbent assay (ELISA) 7 -- 2.9 Statistical analysis 7 -- 3. Results 8 -- 3.1 LPS suppressed autophagic flux in BV-2 and primary microglia 8 -- 3.2 Caffeine induced autophagy in BV-2 and primary microglia 10 -- 3.3 Autophagy inhibition blocked caffeine-induced autophagy and anti-apoptotic effect 11 -- 3.4 Caffeine rescued LPS-induced autophagic suppression and ameliorated LPS-induced inflammation 12 -- 3.5 Ablation of autophagy blocked caffeine’s anti-inflammatory effect 13 -- 4. Discussion 15 -- 5. Figure legends 17 -- 6. Figures 19 -- Reference 27
URI
http://dgist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002294169
http://hdl.handle.net/20.500.11750/1431
DOI
10.22677/thesis.2294169
Degree
Master
Department
Brain and Cognitive Sciences
University
DGIST
Files:
Collection:
Brain and Cognitive SciencesThesesMaster


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