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Characterization of fiber-optic light delivery and light-induced temperature changes in a rodent brain for precise optogenetic neuromodulation

Title
Characterization of fiber-optic light delivery and light-induced temperature changes in a rodent brain for precise optogenetic neuromodulation
Authors
Shin, Y[Shin, Younghoon]Yoo, M[Yoo, Minsu]Kim, HS[Kim, Hyung-Sun]Nam, SK[Nam, Sung-Ki]Kim, HI[Kim, Hyoung-Ihl]Lee, SK[Lee, Sun-Kyu]Kim, S[Kim, Sohee]Kwon, HS[Kwon, Hyuk-Sang]
DGIST Authors
Kim, S[Kim, Sohee]
Issue Date
2016-11-01
Citation
Biomedical Optics Express, 7(11), 4450-4471
Type
Article
Article Type
Article
Keywords
Characterization of FibersLight IntensityLight PropagationLight Propagation in TissueLight Propagation in TissuesMonte-Carlo MethodPhoton MigrationPhotothermal EffectsTemperature IncreaseTemperature MeasuringTissueTurbid Media
ISSN
2156-7085
Abstract
Understanding light intensity and temperature increase is of considerable importance in designing or performing in vivo optogenetic experiments. Our study describes the optimal light power at target depth in the rodent brain that would maximize activation of light-gated ion channels while minimizing temperature increase. Monte Carlo (MC) simulations of light delivery were used to provide a guideline for suitable light power at a target depth. In addition, MC simulations with the Pennes bio-heat model using data obtained from measurements with a temperature-measuring cannula having 12.3 mV/°C of thermoelectric sensitivity enabled us to predict tissue heating of 0.116 °C/mW on average at target depth of 563 μm and specifically, a maximum mean plateau temperature increase of 0.25 °C/mW at 100 μm depth for 473 nm light. Our study will help to improve the design and performance of optogenetic experiments while avoiding potential over-and underillumination. © 2016 Optical Society of America.
URI
http://hdl.handle.net/20.500.11750/1538
DOI
10.1364/BOE.7.004450
Publisher
OSA - The Optical Society
Related Researcher
  • Author Kim, So Hee Neural Interfaces & MicroSystems Lab
  • Research Interests Neural interface; Neural stimulation; Bio MEMS; Stretchable electronics; Numerical simulation of implant-body interactions
Files:
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Collection:
Robotics EngineeringETC1. Journal Articles


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