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Analysis of Phosphoinositide-Binding Properties and Subcellular Localization of GFP-Fusion Proteins
- Analysis of Phosphoinositide-Binding Properties and Subcellular Localization of GFP-Fusion Proteins
- Jun, YW[Jun, Yong-Woo]; Kim, S[Kim, Sangyeol]; Kim, KH[Kim, Kun-Hyung]; Lee, JA[Lee, Jin-A]; Lim, CS[Lim, Chae-Seok]; Chang, I[Chang, Iksoo]; Suh, BC[Suh, Byung-Chang]; Kaang, BK[Kaang, Bong-Kiun]; Jang, DJ[Jang, Deok-Jin]
- DGIST Authors
- Chang, I[Chang, Iksoo]; Suh, BC[Suh, Byung-Chang]
- Issue Date
- Lipids, 50(4), 427-436
- Article Type
- Amino Acid Sequence; Animal; Animals; Aplysia; Aplysia Sec7; Chemical Structure; Cytology; Enhanced Green Fluorescent Protein; Genetics; GFP-Fusion Protein; Green Fluorescent Protein; Green Fluorescent Proteins; HEK293 Cell Line; HEK293 Cells; Human; Humans; Hybrid Protein; In Vitro Protein-Phosphoinositide Binding; Metabolism; Models, Molecular; Molecular Genetics; Molecular Sequence Data; Neurite Outgrowth; Phosphatidylinositol; Phosphatidylinositols; Phosphoinositide; PI(3 4 5)P3; Protein Binding; Recombinant Fusion Proteins
- Specific protein-phosphoinositide (PI) interactions are known to play a key role in the targeting of proteins to specific cellular membranes. Investigation of these interactions would be greatly facilitated if GFP-fusion proteins expressed in mammalian cells and used for their subcellular localization could also be employed for in vitro lipid binding. In this study, we found that lysates of cells overexpressing GFP-fusion proteins could be used for in vitro protein-PI binding assays. We applied this approach to examine the PI-binding properties of Aplysia Sec7 protein (ApSec7) and its isoform ApSec7(VPKIS), in which a VPKIS sequence is inserted into the PH domain of ApSec7. EGFP-ApSec7 but not EGFP-ApSec7(VPKIS) did specifically bind to PI(3,4,5)P3 in an in vitro lipid-coated bead assay. Overexpression of EGFP-ApSec7 but not EGFP-ApSec7(VPKIS) did induce neurite outgrowth in Aplysia sensory neurons. Structure modeling analysis revealed that the inserted VPKIS caused misfolding around the PI(3,4,5)P3-binding pocket of ApSec7 and disturbed the binding of PI(3,4,5)P3 to the pleckstrin homology (PH) domain. Our data indicate that plasma membrane localization of EGFP-ApSec7 via the interaction between its PH domain and PI(3,4,5)P3 might play a key role in neurite outgrowth in Aplysia. © 2015 AOCS.
- SPRINGER HEIDELBERG
- Related Researcher
Chang, Ik Soo
Theoretical and Computational Biophysics Laboratory
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