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High-Resolution Live-Cell Imaging and Analysis by Laser Desorption/Ionization Droplet Delivery Mass Spectrometry

Title
High-Resolution Live-Cell Imaging and Analysis by Laser Desorption/Ionization Droplet Delivery Mass Spectrometry
Authors
Lee, JK[Lee, Jae Kyoo]Jansson, ET[Jansson, Erik T.]Nam, HG[Nam, Hong Gil]Zare, RN[Zare, Richard N.]
DGIST Authors
Nam, HG[Nam, Hong Gil]
Issue Date
2016-05-17
Citation
Analytical Chemistry, 88(10), 5453-5461
Type
Article
Article Type
Article
Keywords
Ambient IonizationsAminesAmino AcidsAmino AlcoholsCarbon-Chain LengthCell DeathCellsChain LengthChainsCytologyDesorptionDropsFatty AcidsIonizationLaser BeamsLaser Desorption/IonizationMass SpectrometersMass SpectrometryPhosphatidylcholinePulsed LasersSingle-Cell AnalysisSpectrometric TechniquesSpectrometryTissueTranslational StageTwo-Dimensional MapUltraviolet Lasers
ISSN
0003-2700
Abstract
We have developed a new ambient-ionization mass spectrometric technique named laser desorption/ionization droplet delivery mass spectrometry (LDIDD-MS). LDIDD-MS permits high-resolution, high-sensitivity imaging of tissue samples as well as measurements of both single-cell apoptosis and live-cell exocytosis. A pulsed (15 Hz) UV laser beam (266 nm) is focused on a surface covered with target analytes to trigger their desorption and ionization. A spray of liquid droplets is simultaneously directed onto the laser-focused surface region to capture the ionized analytes and deliver them to a mass spectrometer. The approach of rapid and effective capturing of molecules after laser desorption/ionization allows the limit of detection for the amino acid lysine to be as low as 2 amol under ambient ionization conditions. Two-dimensional maps of the desorbed/ionized species are recorded by moving the sample on an XY translational stage. The spatial resolution for imaging with LDIDD-MS was determined to be 2.4 μm for an ink-printed pattern and 3 μm for mouse brain tissue. We applied LDIDD-MS to single-cell analysis of apoptotic HEK cells. Differences were observed in the profiles of fatty acids and lipids between healthy HEK cells and those undergoing apoptosis. We observed upregulation of phosphatidylcholine (PC) with a relatively shorter carbon chain length and downregulation of PC with a relatively longer carbon chain length. We also applied LDIDD-MS for a real-time direct measurements of live-cell exocytosis. The catecholamine dopamine and trace amines (phenethylamine and tyramine) were detected from live PC12 cells without damaging them. © 2016 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/1640
DOI
10.1021/acs.analchem.6b00881
Publisher
American Chemical Society
Related Researcher
Files:
There are no files associated with this item.
Collection:
New BiologyETC1. Journal Articles


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