Cited 3 time in webofscience Cited 3 time in scopus

Imaging a specific mRNA in pollen with atomic force microscopy

Title
Imaging a specific mRNA in pollen with atomic force microscopy
Authors
Kim, JS[Kim, Jung Sook]Park, YS[Park, Yu Shin]Nam, HG[Nam, Hong Gil]Park, JW[Park, Joon Won]
DGIST Authors
Park, YS[Park, Yu Shin]; Nam, HG[Nam, Hong Gil]
Issue Date
2015
Citation
RSC Advances, 5(24), 18858-18865
Type
Article
Article Type
Article
Keywords
Ammonium TransportersArabidopsisAtomic Force MicroscopyCellular FunctionDendron MoleculesFacile DetectionInteraction ForcesIntracellular LocalizationMobile SecurityMoleculesNucleic AcidsOligonucleotidesSingle Molecule Level
ISSN
2046-2069
Abstract
Intracellular localization of RNA is important in regulating various cellular functions. Here, we determined the intracellular localization of the ammonium transporter (AtAMT1;4) mRNA in the sperm cells of Arabidopsis pollen by atomic force microscopy (AFM). The AFM probe was modified with cone-shaped dendron molecules, and an oligonucleotide complementary to the mRNA was attached to the apex. This configuration allowed facile detection of the mRNA within a sectioned pollen cell at the single-molecule level by measuring the mRNA-oligonucleotide interaction force. The AtAMT1;4 mRNA was largely localized in the nucleus rather than the cytosol of the sperm cells. © The Royal Society of Chemistry. 2015.
URI
http://hdl.handle.net/20.500.11750/1670
DOI
10.1039/c5ra00199d
Publisher
Royal Society of Chemistry
Related Researcher
Files:
There are no files associated with this item.
Collection:
New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
New BiologyETC1. Journal Articles


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