Cited 2 time in webofscience Cited 2 time in scopus

Imaging a specific mRNA in pollen with atomic force microscopy

Title
Imaging a specific mRNA in pollen with atomic force microscopy
Authors
Kim, JS[Kim, Jung Sook]Park, YS[Park, Yu Shin]Nam, HG[Nam, Hong Gil]Park, JW[Park, Joon Won]
DGIST Authors
Park, YS[Park, Yu Shin]; Nam, HG[Nam, Hong Gil]
Issue Date
2015
Citation
RSC Advances, 5(24), 18858-18865
Type
Article
Article Type
Article
Keywords
Ammonium TransportersArabidopsisAtomic Force MicroscopyCellular FunctionDendron MoleculesFacile DetectionInteraction ForcesIntracellular LocalizationMobile SecurityMoleculesNucleic AcidsOligonucleotidesSingle Molecule Level
ISSN
2046-2069
Abstract
Intracellular localization of RNA is important in regulating various cellular functions. Here, we determined the intracellular localization of the ammonium transporter (AtAMT1;4) mRNA in the sperm cells of Arabidopsis pollen by atomic force microscopy (AFM). The AFM probe was modified with cone-shaped dendron molecules, and an oligonucleotide complementary to the mRNA was attached to the apex. This configuration allowed facile detection of the mRNA within a sectioned pollen cell at the single-molecule level by measuring the mRNA-oligonucleotide interaction force. The AtAMT1;4 mRNA was largely localized in the nucleus rather than the cytosol of the sperm cells. © The Royal Society of Chemistry. 2015.
URI
http://hdl.handle.net/20.500.11750/1670
DOI
10.1039/c5ra00199d
Publisher
Royal Society of Chemistry
Related Researcher
  • Author Nam, Hong Gil CBRG(Complex Biology Research Group)
  • Research Interests Plant Senescence and Life History; Systems Biology; Complexbiology
Files:
There are no files associated with this item.
Collection:
Center for Core Research Facilities1. Journal Articles
New BiologyCBRG(Complex Biology Research Group)1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE