Cited 9 time in webofscience Cited 12 time in scopus

The homeodomain-leucine zipper ATHB23, a phytochrome B-interacting protein, is important for phytochrome B-mediated red light signaling

Title
The homeodomain-leucine zipper ATHB23, a phytochrome B-interacting protein, is important for phytochrome B-mediated red light signaling
Authors
Choi, H[Choi, Hyunmo]Jeong, S[Jeong, Suyeong]Kim, DS[Kim, Dong Su]Na, HJ[Na, Hyung Jin]Ryu, JS[Ryu, Jong Sang]Lee, SS[Lee, Seung Sik]Nam, HG[Nam, Hong Gil]Lim, PO[Lim, Pyung Ok]Woo, HR[Woo, Hye Ryun]
DGIST Authors
Choi, H[Choi, Hyunmo]; Jeong, S[Jeong, Suyeong]; Nam, HG[Nam, Hong Gil]Lim, PO[Lim, Pyung Ok]Woo, HR[Woo, Hye Ryun]
Issue Date
2014-02
Citation
Physiologia Plantarum, 150(2), 308-320
Type
Article
Article Type
Article
Keywords
ArabidopsisArabidopsis ProteinArabidopsis ProteinsATHB23 Protein, ArabidopsisCotyledonFluorescenceGeneticsGerminationGreen Fluorescent ProteinGreen Fluorescent ProteinsGrowth, Development and AgingHomeodomain ProteinHomeodomain ProteinsHypocotylLeucine Zipper ProteinLeucine ZippersLightLight Signal TransductionMass SpectrometryMetabolismMutationPhototransductionPHYB Protein, ArabidopsisPhytochrome BPlant GrowthPlants, Genetically ModifiedProtein BindingRadiation ExposureSeedlingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTransgenic Plant
ISSN
0031-9317
Abstract
Phytochromes are red (R)/far-red (FR) photoreceptors that are central to the regulation of plant growth and development. Although it is well known that photoactivated phytochromes are translocated into the nucleus where they interact with a variety of nuclear proteins and ultimately regulate genome-wide transcription, the mechanisms by which these photoreceptors function are not completely understood. In an effort to enhance our understanding of phytochrome-mediated light signaling networks, we attempted to identify novel proteins interacting with phytochrome B (phyB). Using affinity purification in Arabidopsis phyB overexpressor, coupled with mass spectrometry analysis, 16 proteins that interact with phyB in vivo were identified. Interactions between phyB and six putative phyB-interacting proteins were confirmed by bimolecular fluorescence complementation (BiFC) analysis. Involvement of these proteins in phyB-mediated signaling pathways was also revealed by physiological analysis of the mutants defective in each phyB-interacting protein. We further characterized the athb23 mutant impaired in the homeobox protein 23 (ATHB23) gene. The athb23 mutant displayed altered hypocotyl growth under R light, as well as defects in phyB-dependent seed germination and phyB-mediated cotyledon expansion. Taken together, these results suggest that the ATHB23 transcription factor is a novel component of the phyB-mediated R light signaling pathway. © 2013 Scandinavian Plant Physiology Society.
URI
http://hdl.handle.net/20.500.11750/1683
DOI
10.1111/ppl.12087
Publisher
Wiley Blackwell
Related Researcher
Files:
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Collection:
ETCETC
New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
ETC1. Journal Articles


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