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Human periodontal ligament stem cells suppress T-cell proliferation via down-regulation of non-classical major histocompatibility complex-like glycoprotein CD1b on dendritic cells

Title
Human periodontal ligament stem cells suppress T-cell proliferation via down-regulation of non-classical major histocompatibility complex-like glycoprotein CD1b on dendritic cells
Authors
Shin, C[Shin, C.]Kim, M[Kim, M.]Han, JA[Han, J. -A.]Choi, B[Choi, B.]Hwang, D[Hwang, D.]Do, Y[Do, Y.]Yun, JH[Yun, J. -H.]
DGIST Authors
Hwang, D[Hwang, D.]
Issue Date
2017-02
Citation
Journal of Periodontal Research, 52(1), 135-146
Type
Article
Article Type
Article
Keywords
GlycoproteinsImmunomodulationMajor Histocompatibility ComplexMesenchymal Stem CellsPeriodontal DiseasePorphyromonas Gingivalis
ISSN
0022-3484
Abstract
Background and Objective: Periodontal ligament stem cells (PDLSCs) from the periodontal ligament tissue were recently identified as mesenchymal stem cells (MSCs). The capabilities of PDLSCs in periodontal tissue or bone regeneration have been reported, but their immunomodulatory role in T-cell immune responses via dendritic cells (DCs), known as the most potent antigen-presenting cell, has not been studied. The aim of this study is to understand the immunological function of homogeneous human STRO-1+CD146+ PDLSCs in DC-mediated T-cell immune responses to modulate the periodontal disease process. Material and Methods: We utilized highly purified (> 95%) human STRO-1+CD146+ PDLSCs and human bone marrow mesenchymal stem cells (BMSCs). Each stem cell was co-cultured with human monocyte-derived DCs in the presence of lipopolysaccharide isolated from Porphyromonas gingivalis, a major pathogenic bacterium responsible for periodontal disease, in vitro to examine the immunological effect of each stem cell on DCs and DC-mediated T-cell proliferation. Results: We discovered that STRO-1+CD146+ PDLSCs, as well as BMSCs, significantly decreased the level of non-classical major histocompatibility complex glycoprotein CD1b on DCs, resulting in defective T-cell proliferation, whereas most human leukocyte antigens and the co-stimulatory molecules CD80 and CD86 in/on DCs were not significantly affected by the presence of BMSCs or STRO-1+CD146+ PDLSCs. Conclusions: This study unveiled an immunomodulatory role of STRO-1+CD146+ PDLSCs in negatively regulating DC-mediated T-cell immune responses, demonstrating their potential to be utilized in promising new stem cell therapies. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
URI
http://hdl.handle.net/20.500.11750/2043
DOI
10.1111/jre.12378
Publisher
Wiley Blackwell
Related Researcher
  • Author Hwang, Dae Hee Systems Biology and Medicine Lab
  • Research Interests Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
Files:
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Collection:
New BiologyETC1. Journal Articles


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