Cited 0 time in webofscience Cited 1 time in scopus

Rare Synaptogenesis-Impairing Mutations in SLITRK5 Are Associated with Obsessive Compulsive Disorder

Title
Rare Synaptogenesis-Impairing Mutations in SLITRK5 Are Associated with Obsessive Compulsive Disorder
Authors
Song, M[Song, Minseok]Mathews, CA[Mathews, Carol A.]Stewart, SE[Stewart, S. Evelyn]Shmelkov, SV[Shmelkov, Sergey V.]Mezey, JG[Mezey, Jason G.]Rodriguez-Flores, JL[Rodriguez-Flores, Juan L.]Rasmussen, SA[Rasmussen, Steven A.]Britton, JC[Britton, Jennifer C.]Oh, YS[Oh, Yong-Seok]Walkup, JT[Walkup, John T.]Lee, FS[Lee, Francis S.]Glatt, CE[Glatt, Charles E.]
DGIST Authors
Oh, YS[Oh, Yong-Seok]
Issue Date
2017-01-13
Citation
PLoS ONE, 12(1)
Type
Article
Article Type
Article
ISSN
1932-6203
Abstract
Obsessive compulsive disorder (OCD) is substantially heritable, but few molecular genetic risk factors have been identified. Knockout mice lacking SLIT and NTRK-Like Family, Member 5 (SLITRK5) display OCD-like phenotypes including serotonin reuptake inhibitor-sensitive pathologic grooming, and corticostriatal dysfunction. Thus, mutations that impair SLITRK5 function may contribute to the genetic risk for OCD. We re-sequenced the protein-coding sequence of the human SLITRK5 gene (SLITRK5) in three hundred and seventy seven OCD subjects and compared rare non-synonymous mutations (RNMs) in that sample with similar mutations in the 1000 Genomes database. We also performed in silico assessments and in vitro functional synaptogenesis assays on the Slitrk5 mutations identified. We identified four RNM's among these OCD subjects. There were no significant differences in the prevalence or in silico effects of rare non-synonymous mutations in the OCD sample versus controls. Direct functional testing of recombinant SLITRK5 proteins found that all mutations identified in OCD subjects impaired synaptogenic activity whereas none of the pseudo-matched mutations identified in 1000 Genomes controls had significant effects on SLITRK5 function (Fisher's exact test P = 0.028). These results demonstrate that rare functional mutations in SLITRK5 contribute to the genetic risk for OCD in human populations. They also highlight the importance of biological characterization of allelic effects in understanding genotype-phenotype relationships as there were no statistical differences in overall prevalence or bioinformatically predicted effects of OCD case versus control mutations. Finally, these results converge with others to highlight the role of aberrant synaptic function in corticostriatal neurons in the pathophysiology of OCD. © This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
URI
http://hdl.handle.net/20.500.11750/2050
DOI
10.1371/journal.pone.0169994
Publisher
Public Library of Science
Related Researcher
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE