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L-Met Activates Arabidopsis GLR Ca2+ Channels Upstream of ROS Production and Regulates Stomatal Movement

Title
L-Met Activates Arabidopsis GLR Ca2+ Channels Upstream of ROS Production and Regulates Stomatal Movement
Authors
Kong, D[Kong, Dongdong]Hu, HC[Hu, Heng-Cheng]Okuma, E[Okuma, Eiji]Lee, Y[Lee, Yuree]Lee, HS[Lee, Hui Sun]Munemasa, S[Munemasa, Shintaro]Cho, D[Cho, Daeshik]Ju, C[Ju, Chuanli]Pedoeim, L[Pedoeim, Leah]Rodriguez, B[Rodriguez, Barbara]Wang, J[Wang, Juan]Im, W[Im, Wonpil]Murata, Y[Murata, Yoshiyuki]Pei, ZM[Pei, Zhen-Ming]Kwak, JM[Kwak, June M.]
DGIST Authors
Kwak, JM[Kwak, June M.]
Issue Date
2016-12-06
Citation
Cell Reports, 17(10), 2553-2561
Type
Article
Article Type
Article
Keywords
Ca2+ ChannelCa2+ DeficiencyGlutamate Receptor HomologsGuard CellL-MethionineReactive Oxygen Species (ROS)Stomatal Movement
ISSN
2211-1247
Abstract
Plant glutamate receptor homologs (GLRs) have long been proposed to function as ligand-gated Ca2+ channels, but no in planta evidence has been provided. Here, we present genetic evidence that Arabidopsis GLR3.1 and GLR3.5 form Ca2+ channels activated by L-methionine (L-Met) at physiological concentrations and regulate stomatal apertures and plant growth. The glr3.1/3.5 mutations resulted in a lower cytosolic Ca2+ level, defective Ca2+-induced stomatal closure, and Ca2+-deficient growth disorder, all of which involved L-Met. Patch-clamp analyses of guard cells showed that GLR3.1/3.5 Ca2+ channels are activated specifically by L-Met, with the activation abolished in glr3.1/3.5. Moreover, GLR3.1/3.5 Ca2+ channels are distinct from previously characterized ROS-activated Ca2+ channels and act upstream of ROS, providing Ca2+ transients necessary for the activation of NADPH oxidases. Our data indicate that GLR3.1/3.5 constitute L-Met-activated Ca2+ channels responsible for maintaining basal [Ca2+]cyt, play a pivotal role in plant growth, and act upstream of ROS, thereby regulating stomatal aperture. © 2016 Institute for Basic Science / DGIST
URI
http://hdl.handle.net/20.500.11750/2105
DOI
10.1016/j.celrep.2016.11.015
Publisher
Cell Press
Related Researcher
  • Author Kwak, June Myoung KWAK_LAB(Lab of Cell Signailng)
  • Research Interests Calcium Signaling; ABA;ROS Signaling; drought-tolerant plants; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation
Files:
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Collection:
New BiologyETC1. Journal Articles


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