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Integrin-binding elastin-like polypeptide as an in situ gelling delivery matrix enhances the therapeutic efficacy of adipose stem cells in healing full-thickness cutaneous wounds

Title
Integrin-binding elastin-like polypeptide as an in situ gelling delivery matrix enhances the therapeutic efficacy of adipose stem cells in healing full-thickness cutaneous wounds
Authors
Choi, SK[Choi, Seong-Kyoon]Park, JK[Park, Jin-Kyu]Kim, JH[Kim, Jung-Hee]Lee, KM[Lee, Kyeong-Min]Kim, E[Kim, Enjoo]Jeong, KS[Jeong, Kyu-Shik]Jeon, WB[Jeon, Won Bae]
DGIST Authors
Choi, SK[Choi, Seong-Kyoon]; Park, JK[Park, Jin-Kyu]; Kim, JH[Kim, Jung-Hee]; Lee, KM[Lee, Kyeong-Min]; Kim, E[Kim, Enjoo]
Issue Date
2016-09-10
Citation
Journal of Controlled Release, 237, 89-100
Type
Article
Article Type
Article
Keywords
Adipose Stem CellsAngiogenesisCellsCytologyElastinElastin-Like PolypeptideElastin-Like PolypeptidesEnzyme InhibitionGlycoproteinsNative Extracellular MatrixNeo-VascularizationPolypeptidesRe-EpithelializationRepairSignal TransductionStem Cell SurvivalStem CellsTherapeutic EfficacyTissueTissue RegenerationWound Healing
ISSN
0168-3659
Abstract
One crucial issue in stem cell therapy used for tissue repair is often the lack of selective carriers to deliver stem cells to the site of injury where the native extracellular matrix is pathologically damaged or lost. Therefore, it is necessary to develop a biomaterial that is permissive to stem cells and is suitable to replace injured or missing matrix. The major aim of this study is to investigate the potential of an RGD-containing elastin-like polypeptide (REP) with the structure TGPG[VGRGD(VGVPG)6]20WPC to engraft adipose stem cells (ASC) to full-thickness excisional wounds in mice. We implanted REP into the wound defects via body temperature-induced in situ aggregation. Engrafted REP exhibited a half-life of 2.6 days in the wounds and did not elicit any pathological immune responses. REP itself significantly accelerated wound closure and reepithelialization and upregulated the expression of dermal tissue components. A combined administration of REP and ASC formed a hydrogel-like ASC/REP composite, which provided better neovascularization than the use of ASCs alone and increased the viability of transplanted ASC, improving overall wound healing. In vitro and in vivo mechanistic investigations suggested that REP enhances ASC survival at least in part via the Fak/Src adhesion-induced upregulation of Mek/Erk and PI3K/Akt survival pathways. We conclude that REP is a promising therapeutic agent for the improvement of stem cell-based therapy for enhanced tissue regeneration and repair. © 2016 Elsevier B.V.
URI
http://hdl.handle.net/20.500.11750/2200
DOI
10.1016/j.jconrel.2016.07.006
Publisher
Elsevier B.V.
Files:
There are no files associated with this item.
Collection:
Division of Nano∙Energy Convergence Research1. Journal Articles
ETC1. Journal Articles


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