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Synthesis of one-dimensional gold nanostructures and the electrochemical application of the nanohybrid containing functionalized graphene oxide for cholesterol biosensing

Title
Synthesis of one-dimensional gold nanostructures and the electrochemical application of the nanohybrid containing functionalized graphene oxide for cholesterol biosensing
Authors
Nandini, S[Nandini, Seetharamaiah]Nalini, S[Nalini, Seetharamaiah]Reddy, MBM[Reddy, M. B. Madhusudana]Suresh, GS[Suresh, Gurukar Shivappa]Melo, JS[Melo, Jose Savio]Niranjana, P[Niranjana, Pathappa]Sanetuntikul, J[Sanetuntikul, Jakkid]Shanmugam, S[Shanmugam, Sangaraju]
DGIST Authors
Sanetuntikul, J[Sanetuntikul, Jakkid]; Shanmugam, S[Shanmugam, Sangaraju]
Issue Date
2016-08
Citation
Bioelectrochemistry, 110, 79-90
Type
Article
Article Type
Article
Keywords
Beta-DiphenylalanineBiosensorBiosensorsChemical SensorsCholesterolCholesterol OxidaseChronoamperometryControlled StudyCyclic PotentiometryCyclic VoltammetryDirect Electron TransferDispersionElectro-Chemical Impedance Spectroscopy (EIS)Electrochemical ApplicationsElectrochemical BiosensorElectrodesEnergy Dispersive Analysis of X-RaysEnzyme ElectrodesEnzyme ImmobilizationFunctionalized GrapheneFunctionalized Graphene OxideGoldGold NanoparticleGold NanostrucutresGrapheneGraphene OxideHybridMorphologyMorphology and CompositionNano-StructuresPeptide NanotubesScanning Electron MicroscopySynthesisSynthesis (Chemical)Ultrasonic ApplicationsUltrasound IrradiationVoltage RegulatorsX Ray DiffractionYarn
ISSN
1567-5394
Abstract
This manuscript reports a new approach for the synthesis of one dimensional gold nanostructure (AuNs) and its application in the development of cholesterol biosensor. Au nanostructures have been synthesized by exploiting β-diphenylalanine (β-FF) as an sacrificial template, whereas the Au nanoparticles (AuNPs) were synthesized by ultrasound irradiation. X-ray diffractometer (XRD), scanning electron microscope (SEM) and energy dispersive analysis of X-rays (EDAX) have been employed to characterize the morphology and composition of the prepared samples. With the aim to develop a highly sensitive cholesterol biosensor, cholesterol oxidase (ChOx) was immobilized on AuNs which were appended on the graphite (Gr) electrode via chemisorption onto thiol-functionalized graphene oxide (GO-SH). This Gr/GO-SH/AuNs/ChOx biosensor has been characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy and chronoamperometry. CV results indicated a direct electron transfer between the enzyme and the electrode surface. A new potentiostat intermitant titration technique (PITT) has been studied to determine the diffusion coefficient and maxima potential value. The proposed biosensor showed rapid response, high sensitivity, wide linear range and low detection limit. Furthermore, our AuNs modified electrode showed excellent selectivity, repeatability, reproducibility and long term stability. The proposed electrode has also been used successfully to determine cholesterol in serum samples. © 2016 Elsevier B.V.
URI
http://hdl.handle.net/20.500.11750/2225
DOI
10.1016/j.bioelechem.2016.03.006
Publisher
Elsevier
Related Researcher
Files:
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Collection:
Energy Science and EngineeringAdvanced Energy Materials Laboratory1. Journal Articles


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