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Characterization of a novel antibody immobilization combining protein G with parylene-H for surface plasmon resonance immunosensors

Title
Characterization of a novel antibody immobilization combining protein G with parylene-H for surface plasmon resonance immunosensors
Authors
Shin, EJ[Shin, Eun Jung]Lee, WG[Lee, Won Gu]Moon, SJ[Moon, Sang Jun]
DGIST Authors
Shin, EJ[Shin, Eun Jung]; Moon, SJ[Moon, Sang Jun]
Issue Date
2016-08
Citation
Microsystem Technologies, 22(8), 2093-2099
Type
Article
Article Type
Article
Keywords
AntibodiesAntibody ImmobilizationChemical DetectionCombinatorial ApproachDetection and DiagnosisDetection PerformanceDiagnosisDiagnostic ApplicationsModified SurfacesPlasmonsProof of ConceptProteinsSignal DetectionSurface Plasmon ResonanceSurface Plasmon Resonance Immunosensors
ISSN
0946-7076
Abstract
Here we present a method for selectively and efficiently immobilizing antibodies to enhance the detection performance of surface plasmon resonance immune-sensors (SPRIs) for diagnostic applications. To improve the performance of antibody arrays, protein G was used as antibody-selective linkage layer with aldehyde functionalized poly-(para-xylylene) film. To estimate the efficiency of antibody immobilization, immunoglobulin G (IgG) was measured using the anti-IgG immobilized SPRIs. To demonstrate the proof-of-concept validation, the signal detected from the IgG using parylene-H film was compared with that of a combination of parylene-H and protein G in SPRIs. The results showed that the detection of IgG on the immobilized anti-IgG layer using the combination of parylene-H and protein G has a larger change of signal than that of using parylene-H layer. These results also imply that the anti-IgG was densely and efficiently immobilized on the modified surface with the linkage layer in a combination with parylene-H and protein G. Therefore, we believe that this combinatorial approach could selectively immobilize the antibodies, and also be applied for detection and diagnosis of immune diseases in the field of many SPRIs applications. © 2015, Springer-Verlag Berlin Heidelberg.
URI
http://hdl.handle.net/20.500.11750/2228
DOI
10.1007/s00542-015-2534-3
Publisher
Springer
Files:
There are no files associated with this item.
Collection:
Robotics EngineeringCybernetics Laboratory1. Journal Articles
Robotics EngineeringETC1. Journal Articles


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