Cited 7 time in webofscience Cited 8 time in scopus

Cell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of Cell Mechanics

Title
Cell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of Cell Mechanics
Authors
Hwang, JY[Hwang, Jae Youn]Kim, J[Kim, Jihun]Park, JM[Park, Jin Man]Lee, C[Lee, Changyang]Jung, H[Jung, Hayong]Lee, J[Lee, Jungwoo]Shung, KK[Shung, K. Kirk]
DGIST Authors
Hwang, JY[Hwang, Jae Youn]; Kim, J[Kim, Jihun]; Park, JM[Park, Jin Man]
Issue Date
2016-06-08
Citation
Scientific Reports, 6
Type
Article
Article Type
Article
ISSN
2045-2322
Abstract
We demonstrate a noncontact single-beam acoustic trapping method for the quantification of the mechanical properties of a single suspended cell with label-free. Experimentally results show that the single-beam acoustic trapping force results in morphological deformation of a trapped cell. While a cancer cell was trapped in an acoustic beam focus, the morphological changes of the immobilized cell were monitored using bright-field imaging. The cell deformability was then compared with that of a trapped polystyrene microbead as a function of the applied acoustic pressure for a better understanding of the relationship between the pressure and degree of cell deformation. Cell deformation was found to become more pronounced as higher pressure levels were applied. Furthermore, to determine if this acoustic trapping method can be exploited in quantifying the cell mechanics in a suspension and in a non-contact manner, the deformability levels of breast cancer cells with different degrees of invasiveness due to acoustic trapping were compared. It was found that highly-invasive breast cancer cells exhibited greater deformability than weakly-invasive breast cancer cells. These results clearly demonstrate that the single-beam acoustic trapping technique is a promising tool for non-contact quantitative assessments of the mechanical properties of single cells in suspensions with label-free.
URI
http://hdl.handle.net/20.500.11750/2260
DOI
10.1038/srep27238
Publisher
Nature Publishing Group
Related Researcher
  • Author Hwang, Jae Youn MBIS(Multimodal Biomedical Imaging and System) Laboratory
  • Research Interests
Files:
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Collection:
Information and Communication EngineeringETC1. Journal Articles
Information and Communication EngineeringETC1. Journal Articles


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