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The fate of extrachromosomal DNAs in the progeny of plastid-transformed tobacco plants

Title
The fate of extrachromosomal DNAs in the progeny of plastid-transformed tobacco plants
Authors
Min, SR[Min, Sung Ran]Jung, SH[Jung, Seo Hee]Liu, JR[Liu, Jang R.]Jeong, WJ[Jeong, Won-Joong]
DGIST Authors
Liu, JR[Liu, Jang R.]
Issue Date
2015-11
Citation
Plant Biotechnology Reports, 9(6), 431-442
Type
Article
Article Type
Article
Keywords
Extrachromosomal DnaHomologous RecombinationPlastid TransformationTobacco
ISSN
1863-5466
Abstract
Plastid transformation is conducted by homologous recombination. Plastid transformation using a vector-containing promoter and/or terminator sequences homologous to the plastid DNA for transgene expression often results in the generation of unintended extrachromosomal DNA molecules derived from the initial plastid DNA molecule via intramolecular recombination. All the extrachromosomal DNA molecules, including those lacking the ori sequence, found in the T0 plastid-transformed tobacco plants were still detected in the T3 plants. These results suggest that the extrachromosomal DNA molecules are newly generated from the initially transformed plastid DNA that is transmitted to the progeny during each generation. In addition, inward, outward, and overlap extension PCR of the extrachromosomal DNA molecules confirmed that these molecules were circular. © 2015, Korean Society for Plant Biotechnology and Springer Japan.
URI
http://hdl.handle.net/20.500.11750/2327
DOI
10.1007/s11816-015-0380-5
Publisher
Springer
Files:
There are no files associated with this item.
Collection:
School of Undergraduate Studies1. Journal Articles


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