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Spirally-patterned pinhole arrays for long-term fluorescence cell imaging

Title
Spirally-patterned pinhole arrays for long-term fluorescence cell imaging
Authors
Koo, BU[Koo, Bon Ung]Kang, Y[Kang, YooNa]Moon, S[Moon, SangJun]Lee, WG[Lee, Won Gu]
DGIST Authors
Kang, Y[Kang, YooNa]; Moon, S[Moon, SangJun]
Issue Date
2015
Citation
Analyst, 140(21), 7373-7381
Type
Article
Article Type
Article
Keywords
ApoptosisCell CountCell NucleusCell SurvivalChemistryCost-Benefit AnalysisEquipment DesignFluorescenceFluorescence MicroscopyFluorescent DyeFluorescent DyesHela Cell LineHela CellsHumanHumansMetabolismMicroscopy, FluorescenceNecrosisProceduresUltra-Violet RadiationUltraviolet Rays
ISSN
0003-2654
Abstract
Fluorescence cell imaging using a fluorescence microscope is an extensively used technique to examine the cell nucleus, internal structures, and other cellular molecules with fluorescence response time and intensity. However, it is difficult to perform high resolution cell imaging for a long period of time with this technique due to necrosis and apoptosis depending on the type and subcellular location of the damage caused by phototoxicity. A large number of studies have been performed to resolve this problem, but researchers have struggled to meet the challenge between cellular viability and image resolution. In this study, we employ a specially designed disc to reduce cell damage by controlling total fluorescence exposure time without deterioration of the image resolution. This approach has many advantages such as, the apparatus is simple, cost-effective, and easily integrated into the optical pathway through a conventional fluorescence microscope. © 2015 The Royal Society of Chemistry.
URI
http://hdl.handle.net/20.500.11750/2362
DOI
10.1039/c5an01423a
Publisher
Royal Society of Chemistry
Files:
There are no files associated with this item.
Collection:
Robotics EngineeringCybernetics Laboratory1. Journal Articles


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