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Surface immobilization of MEPE peptide onto HA/ss-TCP ceramic particles enhances bone regeneration and remodeling
- Surface immobilization of MEPE peptide onto HA/ss-TCP ceramic particles enhances bone regeneration and remodeling
- Acharya, B[Acharya, Bodhraj]; Chun, SY[Chun, So-Young]; Kim, SY[Kim, Shin-Yoon]; Moon, C[Moon, Cheil]; Shin, HI[Shin, Hong-In]; Park, EK[Park, Eui Kyun]
- DGIST Authors
- Moon, C[Moon, Cheil]
- Issue Date
- Journal of Biomedical Materials Research Part B: Applied Biomaterials, 100B(3), 841-849
- Article Type
- Acid Phosphatase Tartrate Resistant Isoenzyme; Animal Experiment; Animal Model; Animals; Bioactive Peptides; Bone; Bone Area; Bone Defect; Bone Mineralization; Bone Regeneration; Bone Remodeling; Calcium Phosphate; Calcium Phosphate Ceramic; Calcium Phosphate Ceramics; Calcium Phosphates; Calvaria; Calvarial Defects; Carboxy Terminal Sequence; Cell Differentiation; Cells, Cultured; Ceramic Particle; Covalent Bond; Disease Models, Animal; Durapatite; Extracellular; Extracellular Matrix Proteins; FT-IR; Glycoproteins; Hematopoietic Stem Cell; Human; Human Cell; Humans; Hydroxyapatite; Immobilized Proteins; In-Situ; Infrared Spectroscopy; Male; Matrix Extracellular Phosphoglycoprotein; MEPE and Bone Marrow Stem Cell; Mice; Mice, Inbred ICR; Micro-Computed Tomography; Micro CT; Mouse; Non-Human; Osteoblast; Osteoblast Differentiation; Osteoblasts; Osteoclast; Osteoclasts; Osteogenic Potential; Peptide Immobilization; Peptides; Phosphatases; Phosphoproteins; Protein Immobilization; Scaffolds (Biology); Skull Fractures; Stem Cells; Surface Immobilization; Surface Modification; Surface Treatment; Tri-Calcium Phosphates; X Ray Photoelectron Spectroscopy
- Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/ β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS) 2, and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling. © 2012 WILEY PERIODICALS, INC.
- Wiley Blackwell
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