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Simultaneous detection of staphylococcus aureus, salmonella enterica subsp., vibrio parahaemolyticus by multiplex polymerase chain reaction

Title
Simultaneous detection of staphylococcus aureus, salmonella enterica subsp., vibrio parahaemolyticus by multiplex polymerase chain reaction
Authors
Jeong, Y.S.[Jeong, Yoo Seok]Jung, H.K.[Jung, Hee Kyoung]Jeon, W.-B.[Jeon, Won Bae]Seo, H.-J.[Seo, Hwa Jung]Hong, J.-H.[Hong, Joo Heon]
DGIST Authors
Jeon, W.-B.[Jeon, Won Bae]; Seo, H.-J.[Seo, Hwa Jung]
Issue Date
2010
Citation
Journal of the Korean Society of Food Science and Nutrition, 39(4), 595-601
Type
Article
Article Type
Article
Keywords
Bacillus CereusBacteria (Microorganisms)Candida AlbicansEscherichia ColiListeria MonocytogenesMultiplex PcrProteus VulgarisSalmonella EntericaSalmonella Enterica Subsp.Shigella SonneiStaphylococcus AureusStreptococcus PyogenesVibrio Parahaemolyticus
ISSN
1226-3311
Abstract
This study was conducted to detect and identify Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella enterica subsp. using simultaneous multiplex polymerase chain reaction (multiplex PCR) assay. 23S rRNA partial gene (S. aureus), tox R gene (V. parahaemolyticus), and inv A gene (S. enterica subsp.) as diagnostic marker gene were suggested, and their amplicon sizes were 482 bp, 368 bp, and 284 bp, respectively. Non specific amplicons by STA-5F/STA-5R primer, ToxR-F/ToxR-R primer, and 139/141 primer were not observed in genomic DNA of pathogen bacteria as Bacillus cereus, Listeria monocytogenes, Escherichia coli, Proteus vulgaris, Streptococcus pyogenes, Candida albicans, and Shigella sonnei. The extracted crude DNA of targeted bacteria was detected as PCR template successfully. The detection limits were 105~104 CFU/mL and 10 pg of purified genomic DNA of S. aureus, V. parahaemolyticus, and S. enterica subsp. by using simultaneous multiplex PCR.
URI
http://hdl.handle.net/20.500.11750/2498
DOI
10.3746/jkfn.2010.39.4.595
Publisher
Korean Society of Food Science and Nutrition
Related Researcher
Files:
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Collection:
ETC1. Journal Articles
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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