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Alternatively activated brain-infiltrating macrophages facilitate recovery from collagenase-induced intracerebral hemorrhage
- Alternatively activated brain-infiltrating macrophages facilitate recovery from collagenase-induced intracerebral hemorrhage
- Min, H[Min, Hyunjung]; Jang, YH[Jang, Yong Ho]; Cho, IH[Cho, Ik-Hyun]; Yu, SW[Yu, Seong-Woon]; Lee, SJ[Lee, Sung Joong]
- DGIST Authors
- Yu, SW[Yu, Seong-Woon]
- Issue Date
- Molecular Brain, 9
- Article Type
- Animal Cell; Animal Experiment; Animal Model; Animal Tissue; Bioaccumulation; Bone Marrow Derived Macrophage; Brain Cell; Brain Damage; Brain Hemorrhage; Brain Injury; Brain Protection; Cell Count; Cell Polarity; Chemokine Receptor CX3CR1; Coculture; Collagenase; Controlled Study; CX3CR1+ Macrophage; Disease Severity; Glia Cell; Hematoma; Immune Response; Macrophage; Macrophage Activation; Macrophage Function; Macrophage Migration; Macrophages; Male; Mannose Receptor; Mouse; Neurologic Disease; Non-Human; Phenotype; Priority Journal; Protein Expression; Wound Healing
- Background: Intracerebral hemorrhage (ICH) is one of the major causes of stroke. After onset of ICH, massive infiltration of macrophages is detected in the peri-hematoma regions. Still, the function of these macrophages in ICH has not been completely elucidated. Results: In a collagenase-induced ICH model, CX3CR1+ macrophages accumulated in the peri-hematoma region. Characterization of these macrophages revealed expression of alternatively activated (M2) macrophage markers. In the macrophage-depleted mice, ICH-induced brain lesion volume was larger and neurological deficits were more severe compared to those of control mice, indicating a protective role of these macrophages in ICH. In the ICH-injured brain, mannose receptor-expressing macrophages increased at a delayed time point after ICH, indicating M2 polarization of the brain-infiltrating macrophages in the brain microenvironment. To explore this possibility, bone marrow-derived macrophages (BMDM) were co-cultured with mouse brain glial cells and then tested for activation phenotype. Upon co-culture with glia, the number of mannose receptor-positive M2 macrophages was significantly increased. Furthermore, treatment with glia-conditioned media increased the number of BMDM of M2 phenotype. Conclusions: In this study, our data suggest that brain-infiltrating macrophages after ICH are polarized to the M2 phenotype by brain glial cells and thereby contribute to recovery from ICH injury. © 2016 Min et al.
- BioMed Central Ltd.
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