Cited 6 time in webofscience Cited 6 time in scopus

Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells

Title
Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells
Authors
Hwang, JY[Hwang, Jae Youn]Yoon, CW[Yoon, Chi Woo]Lim, HG[Lim, Hae Gyun]Park, JM[Park, Jin Man]Yoon, S[Yoon, Sangpil]Lee, J[Lee, Jungwoo]Shung, KK[Shung, K. Kirk]
DGIST Authors
Hwang, JY[Hwang, Jae Youn]; Park, JM[Park, Jin Man]
Issue Date
2015-12
Citation
Ultrasonics, 63, 94-101
Type
Article
Article Type
Article
Keywords
Acoustic TweezersBinsBreast Cancer CellsBreast NeoplasmsBreast TumorCalciumCell ProliferationCell SeparationCell SignalingCellsCytologyDevicesDiseasesEnzyme ActivityEquipment DesignFibronectinFibronectinsHigh-Frequency Ultrasound TransducerHigh Frequency UltrasoundsHumanHumansIntracellular CalciumIntracellular Calcium ElevationMetabolismMicromanipulationMicrosphereMicrospheresMobile SecurityMolecular BiologyPlants (Botany)ProceduresProteinsSignal TransductionSignalingSKBR-3 Breast Cancer CellsTransducerTransducersTumor Cell CultureTumor Cells, CulturedUltrasonic TransducersUltrasonicsUltrasound
ISSN
0041-624X
Abstract
Extracellular matrix proteins such as fibronectin (FNT) play crucial roles in cell proliferation, adhesion, and migration. For better understanding of these associated cellular activities, various microscopic manipulation tools have been used to study their intracellular signaling pathways. Recently, it has appeared that acoustic tweezers may possess similar capabilities in the study. Therefore, we here demonstrate that our newly developed acoustic tweezers with a high-frequency lithium niobate ultrasonic transducer have potentials to study intracellular calcium signaling by FNT-binding to human breast cancer cells (SKBR-3). It is found that intracellular calcium elevations in SKBR-3 cells, initially occurring on the microbead-contacted spot and then eventually spreading over the entire cell, are elicited by attaching an acoustically trapped FNT-coated microbead. Interestingly, they are suppressed by either extracellular calcium elimination or phospholipase C (PLC) inhibition. Hence, this suggests that our acoustic tweezers may serve as an alternative tool in the study of intracellular signaling by FNT-binding activities. © 2015 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/2574
DOI
10.1016/j.ultras.2015.06.017
Publisher
Elsevier
Related Researcher
  • Author Hwang, Jae Youn MBIS(Multimodal Biomedical Imaging and System) Laboratory
  • Research Interests
Files:
There are no files associated with this item.
Collection:
Information and Communication EngineeringETC1. Journal Articles
Information and Communication EngineeringETC1. Journal Articles


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