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Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells
- Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells
- Hwang, JY[Hwang, Jae Youn]; Yoon, CW[Yoon, Chi Woo]; Lim, HG[Lim, Hae Gyun]; Park, JM[Park, Jin Man]; Yoon, S[Yoon, Sangpil]; Lee, J[Lee, Jungwoo]; Shung, KK[Shung, K. Kirk]
- DGIST Authors
- Hwang, JY[Hwang, Jae Youn]; Park, JM[Park, Jin Man]
- Issue Date
- Ultrasonics, 63, 94-101
- Article Type
- Acoustic Tweezers; Bins; Breast Cancer Cells; Breast Neoplasms; Breast Tumor; Calcium; Cell Proliferation; Cell Separation; Cell Signaling; Cells; Cytology; Devices; Diseases; Enzyme Activity; Equipment Design; Fibronectin; Fibronectins; High-Frequency Ultrasound Transducer; High Frequency Ultrasounds; Human; Humans; Intracellular Calcium; Intracellular Calcium Elevation; Metabolism; Micromanipulation; Microsphere; Microspheres; Mobile Security; Molecular Biology; Plants (Botany); Procedures; Proteins; Signal Transduction; Signaling; SKBR-3 Breast Cancer Cells; Transducer; Transducers; Tumor Cell Culture; Tumor Cells, Cultured; Ultrasonic Transducers; Ultrasonics; Ultrasound
- Extracellular matrix proteins such as fibronectin (FNT) play crucial roles in cell proliferation, adhesion, and migration. For better understanding of these associated cellular activities, various microscopic manipulation tools have been used to study their intracellular signaling pathways. Recently, it has appeared that acoustic tweezers may possess similar capabilities in the study. Therefore, we here demonstrate that our newly developed acoustic tweezers with a high-frequency lithium niobate ultrasonic transducer have potentials to study intracellular calcium signaling by FNT-binding to human breast cancer cells (SKBR-3). It is found that intracellular calcium elevations in SKBR-3 cells, initially occurring on the microbead-contacted spot and then eventually spreading over the entire cell, are elicited by attaching an acoustically trapped FNT-coated microbead. Interestingly, they are suppressed by either extracellular calcium elimination or phospholipase C (PLC) inhibition. Hence, this suggests that our acoustic tweezers may serve as an alternative tool in the study of intracellular signaling by FNT-binding activities. © 2015 Elsevier B.V. All rights reserved.
- Related Researcher
Hwang, Jae Youn
MBIS(Multimodal Biomedical Imaging and System) Laboratory
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- Information and Communication EngineeringETC1. Journal Articles
Information and Communication EngineeringETC1. Journal Articles
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