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dc.contributor.author Min, Sung Ran -
dc.contributor.author Davarpanah, Seyed Javad -
dc.contributor.author Jung, Seo Hee -
dc.contributor.author Park, Youn-il -
dc.contributor.author Liu, Jang R. -
dc.contributor.author Jeong, Won-Joong -
dc.date.available 2017-07-11T04:41:39Z -
dc.date.created 2017-04-10 -
dc.date.issued 2015-11 -
dc.identifier.issn 1863-5466 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/2584 -
dc.description.abstract We developed a new plastid transformation vector system using the putative replication origin of a minicircular chromosome from the marine dinoflagellate Heterocapsatriquetra. Transplastomic tobacco plants generated with this vector properly expressed the green fluorescent protein (GFP) gene without incorporating it into the plastid genome. To construct the episomal vector, a 610-bp DNA fragment containing the putative replication origin was fused to a dicistronic expression cassette encoding the aminoglycoside 3′-adenyltransferase (aadA) and gfp genes under control of the plastid rrn promoter. The vector was delivered to plastids of tobacco leaf explants by biolistic bombardment. After 8weeks of bombardment, episomal transformant shoots were generated from leaf explants cultured on selection media containing 500mg/L spectinomycin. Fluorescence microscopy and northern blot analysis demonstrated GFP expression in episomal transformant plants. PCR, Southern blot analysis, recovery of episomes, and sequencing analysis showed the vector to be maintained as self-replicating extrachromosomal circular DNA molecules for at least 6months. Using a single construct for all plants, our episomal vector system may offer an advantage over the conventional plastid vector systems, which require species-specific constructs. © 2015, Korean Society for Plant Biotechnology and Springer Japan. -
dc.publisher SPRINGER -
dc.title An episomal vector system for plastid transformation in higher plants -
dc.type Article -
dc.identifier.doi 10.1007/s11816-015-0381-4 -
dc.identifier.scopusid 2-s2.0-84948568684 -
dc.identifier.bibliographicCitation Plant Biotechnology Reports, v.9, no.6, pp.443 - 449 -
dc.subject.keywordAuthor Episome -
dc.subject.keywordAuthor Heterocapsa triquetra -
dc.subject.keywordAuthor Plastid transformation -
dc.subject.keywordAuthor Tobacco -
dc.subject.keywordPlus CHLOROPLAST GENOMES -
dc.subject.keywordPlus DINofLAGELLATE -
dc.subject.keywordPlus ELemENTS -
dc.subject.keywordPlus Episome -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus Gene -
dc.subject.keywordPlus Heterocapsa Triquetra -
dc.subject.keywordPlus Heterocapsatriquetra -
dc.subject.keywordPlus Plastid Transformation -
dc.subject.keywordPlus PROTEIN -
dc.subject.keywordPlus REPLICATION -
dc.subject.keywordPlus RNAS -
dc.subject.keywordPlus SELECTION -
dc.subject.keywordPlus Tobacco -
dc.subject.keywordPlus TRANSPLASTOMIC TOBACCO -
dc.citation.endPage 449 -
dc.citation.number 6 -
dc.citation.startPage 443 -
dc.citation.title Plant Biotechnology Reports -
dc.citation.volume 9 -
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Appears in Collections:
ETC 1. Journal Articles
School of Undergraduate Studies 1. Journal Articles

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