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ADP-ribosylation Factor 6 (ARF6) Bidirectionally Regulates Dendritic Spine Formation Depending on Neuronal Maturation and Activity
- ADP-ribosylation Factor 6 (ARF6) Bidirectionally Regulates Dendritic Spine Formation Depending on Neuronal Maturation and Activity
- Kim, Y[Kim, Yoonju]; Lee, SE[Lee, Sang-Eun]; Park, J[Park, Joohyun]; Kim, M[Kim, Minhyung]; Lee, B[Lee, Boyoon]; Hwang, D[Hwang, Daehee]; Chang, S[Chang, Sunghoe]
- DGIST Authors
- Hwang, D[Hwang, Daehee]
- Issue Date
- Journal of Biological Chemistry, 290(12), 7323-7335
- Article Type
- 4 Aminobutyric Acid A Receptor; Activation Analysis; Activity-Dependent; Adenosine Diphosphate Ribosylation Factor; Adenosine Diphosphate Ribosylation Factor 6; ADP-Ribosylation Factor 6; ADP-Ribosylation Factors; Animal; Animal Cell; Animals; ARF6 Gene; Base Sequence; Cell Activity; Cell Count; Cell Culture; Cell Density; Cell Maturation; Cell Structure; Cell Viability; Cells, Cultured; Chemical Activation; Controlled Study; Cytology; Dendritic Spine; Dendritic Spines; Down-Regulation; Dynamic Changes; Embryology; Fetus; Gene Expression; Gene Expression Profiling; Gene Expression Regulation; Genetics; Hippocampus; Long-Term Potentiations; Long Term Potentiation; Microarray Analysis; Nerve Cell; Nerve Cell Differentiation; Nerve Cell Plasticity; Neuronal Activities; Neuronal Morphology; Neurons; Non-Human; Nucleotide Sequence; P21 Activated Kinase 1; Phosphatidic Acid; Phosphatidylinositol 4 Phosphate Kinase; Phosphatidylinositol 4,5 Bisphosphate; Phospholipase D; Physiology; Picrotoxin; Priority Journal; Protein Expression; Protein Function; Rac1 Protein; Rat; Rats; Rats, Sprague-Dawley; Rho Guanine Nucleotide Dissociation Inhibitor; RhoA Guanine Nucleotide Binding Protein; RNA, Small Interfering; Signal Transduction; Small Interfering Rna; Spine Structure; Sprague Dawley Rat; Tetrodotoxin; Transcription Regulation; Upregulation
- Recent studies have reported conflicting results regarding the role of ARF6 in dendritic spine development, but no clear answer for the controversy has been suggested. We found that ADP-ribosylation factor 6 (ARF6) either positively or negatively regulates dendritic spine formation depending on neuronal maturation and activity. ARF6 activation increased the spine formation in developing neurons, whereas it decreased spine density in mature neurons. Genome-wide microarray analysis revealed that ARF6 activation in each stage leads to opposite patterns of expression of a subset of genes that are involved in neuronal morphology. ARF6-mediated Rac1 activation via the phospholipase D pathway is the coincident factor in both stages, but the antagonistic RhoA pathway becomes involved in the mature stage. Furthermore, blocking neuronal activity in developing neurons using tetrodotoxin or enhancing the activity in mature neurons using picrotoxin or chemical long term potentiation reversed the effect of ARF6 on each stage. Thus, activity-dependent dynamic changes in ARF6-mediated spine structures may play a role in structural plasticity of mature neurons. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
- American Society for Biochemistry and Molecular Biology Inc.
- Related Researcher
Hwang, Dae Hee
Systems Biology and Medicine Lab
Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
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