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Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone via NF-kappa B inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage
- Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone via NF-kappa B inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage
- Kim, MJ[Kim, Min Jeong]; Lee, HH[Lee, Hye Hyeon]; Jeong, JW[Jeong, Jin Woo]; Seo, MJ[Seo, Min Jeong]; Kang, BW[Kang, Byoung Won]; Park, JU[Park, Jeong Uck]; Kim, KS[Kim, Kyoung-Sook]; Cho, YS[Cho, Young-Su]; Seo, KI[Seo, Kwon-Il]; Kim, GY[Kim, Gi-Young]; Kim, JI[Kim, Jung-In]; Choi, YH[Choi, Yung Hyun]; Jeong, YK[Jeong, Yong Kee]
- DGIST Authors
- Jeong, JW[Jeong, Jin Woo]
- Issue Date
- Molecular Medicine Reports, 9(4), 1197-1203
- Article Type
- 5-Hydroxy-3,6,7,8,3′,4′-Hexamethoxyflavone; 5 Hydroxy 3,6,7,8,3' ,4' Hexamethoxyflavone; Alga; Animal Cell; Animals; Anti-Inflammatory; Anti-Inflammatory Activity; Anti-Inflammatory Agent; Anti-Inflammatory Agents; Cell Culture; Cell Death; Cell Line; Cell Nucleus; Cell Survival; Confocal Microscopy; Cyclooxygenase 2; Cytokine Production; Cytosol; Cytotoxicity; Dinoprostone; Down-Regulation; Flavone Derivative; Flavones; Gene Expression; Glyceraldehyde 3 Phosphate Dehydrogenase; Hizikia Fusiforme; I Kappa B Alpha; Immunofluorescence; Inducible Nitric Oxide Synthase; Interleukin-1 Beta; Lipopolysaccharide; Lipopolysaccharides; Macrophage; Macrophages; Mice; MTT Assay; NF-Kappa B; Nitric Oxide; Nitric Oxide Synthase Type Iii; Non-Human; nuclear factor-Kappa B; Prostaglandin E2; Protective Agents; Protein Degradation; Reverse Transcriptase-Polymerase Chain Reaction; Signal Transduction; Transcription Factor Rela; Tumor Necrosis Factor-Alpha; Unclassified Drug; Western Blotting
- The anti-inflammatory mechanism of 5-hydroxy-3,6,7,8,3′,4′- hexamethoxyflavone (5HHMF), a polyhydroxyflavone isolated from the marine algae Hizikia fusiforme, was investigated in RAW 264.7 murine macrophage cells. Western blot and reverse transcriptase PCR analyses indicated that adding 5HHMF to cultured cells significantly reduced the production of nitric oxide and prostaglandin E2 and downregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, 5HHMF inhibited the release of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-1β, and decreased the transcriptional levels. In particular, 5HHMF significantly inhibited the LPS-induced nuclear factor-κB (NF-κB) translocation from the cytosol to the nucleus, which was associated with the abrogation of inhibitory IκBα degradation and subsequent decreases in nuclear p65 levels. In conclusion, these results suggested that the anti-inflammatory activities of 5HHMF may be attributed to the inhibition of iNOS, COX-2 and cytokine expression by attenuating NF-κB activation via IκBβ degradation in macrophages.
- Spandidos Publications
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