Cited 9 time in
Cited 10 time in
Amperometric hydrogen peroxide and cholesterol biosensors designed by using hierarchical curtailed silver flowers functionalized graphene and enzymes deposits
- Amperometric hydrogen peroxide and cholesterol biosensors designed by using hierarchical curtailed silver flowers functionalized graphene and enzymes deposits
- Nalini, S[Nalini, Seetharamaiah]; Nandini, S[Nandini, Seetharamaiah]; Shanmugam, S[Shanmugam, Sangaraju]; Neelagund, SE[Neelagund, Shivayogeeswar E.]; Melo, JS[Melo, Jose Savio]; Suresh, GS[Suresh, Gurukar Shivappa]
- DGIST Authors
- Shanmugam, S[Shanmugam, Sangaraju]
- Issue Date
- Journal of Solid State Electrochemistry, 18(3), 685-701
- Article Type
- Activation Energy; Biosensors; Cholesterol; Cholesterol Oxidase; Chronoamperometry; Cyclic Voltammetry; Differential Pulse Voltammetry; Enzyme Immobilization; Functionalized Graphene; Galvanostatic Electrodeposition; Galvanostatics; Graphene; Graphite Electrodes; Horse-Radish Peroxidase; Hydrogen Peroxide; Peroxides; Scanning Electron Microscopy; Silver; Silver Deposits
- Novel flower-like silver particles with triangular plates as building block along with functionalized graphene (straggled sheets) and enzymes horseradish peroxidase (HRP) or cholesterol oxidase (ChOx), were obtained on graphite electrode by galvanostatic electrodeposition method. The morphology of the electrodeposits has been characterized using scanning electron microscopy and energy-dispersive analysis of X-ray. The resulting biosensors named Nf/(HRP-f-graphene-Ag)/Gr and Nf/(ChOx-f-graphene-Ag)/Gr were evaluated for electrochemical activity using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry. Optimization of the interdependent experimental parameters such as pH and temperature were achieved and maintained constant throughout the experiments. An activation energy of 2.5 kJ mol -1 was obtained for Nf/(HRP-f-graphene-Ag)/Gr electrode while Nf/(ChOx-f-graphene-Ag)/Gr showed an activation energy of 2.06 and 3.12 kJ mol-1. Furthermore, the former electrode demonstrated a good linear range of 25 μM to 19.35 mM with rapid response time of 3 s and detection limit of 5 μM for hydrogen peroxide. Similarly, the Nf/(ChOx-f-graphene-Ag)/ Gr electrode revealed a linear range of 0.1-4.5 mM with rapid response time of 3 s and an excellent detection limit of 0.514 mM for cholesterol. Besides this, the Nf/(HRP-f-graphene-Ag)/Gr and Nf/(ChOx-f-graphene-Ag)/Gr electrodes displayed a Michaelis-Menten constant of 0.26 and 0.57 mM, respectively, suggesting high affinity and enzymatic activity. The enhanced performance of biosensors towards detection of substrate and rejection of interferents, provided an evidence for its high anti-interference ability. Additionally the biosensors exhibit long term storage stability and reproducibility with antifouling properties. © 2013 Springer-Verlag Berlin Heidelberg.
- Related Researcher
There are no files associated with this item.
- Energy Science and EngineeringAdvanced Energy Materials Laboratory1. Journal Articles
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.