Cited 13 time in webofscience Cited 12 time in scopus

AMPK ACTIVATION REGULATES NEURONAL STRUCTURE IN DEVELOPING HIPPOCAMPAL NEURONS

Title
AMPK ACTIVATION REGULATES NEURONAL STRUCTURE IN DEVELOPING HIPPOCAMPAL NEURONS
Authors
Ramamurthy, S[Ramamurthy, S.]Chang, E[Chang, E.]Cao, Y[Cao, Y.]Zhu, J[Zhu, J.]Ronnett, GV[Ronnett, G. V.]
DGIST Authors
Ronnett, GV[Ronnett, G. V.]
Issue Date
2014-02-14
Citation
Neuroscience, 259, 13-24
Type
Article
Article Type
Article
Keywords
4′,6-Diamidino-2-Phenylindole5-Aminoimidazole-4-Carboxamide-1-B-D-Ribofuranoside5′-Adenosine Monophosphate-Activated Protein KinaseAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphateADPAge FactorsAICARAminoimidazole CarboxamideAMPAMP-Activated Protein KinasesAMPKAnimal CellAnimalsAnimals, NewbornATPAxonBovine Serum AlbuminBrain-Specific KinaseBrain Nerve CellBRSKBSACalcium/Calmodulin-Dependent Kinase Kinase BetaCaMKK BetaCell EnergyCell StressCells, CulturedControlled StudyDAPIDays In VitroDendriteDendritesDevelopmentDIVDPBSDulbecco&aposs Phosphate-Buffered SalineEmbryo, MammalianEnzyme ActivationEnzyme ActivityEnzyme RegulationEnzyme StructureEnzyme SubunitFemaleGene Expression Regulation, DevelopmentalGlycogen Synthase Kinase-3BetaGSK-3 BetaHank&aposs Balanced Salt SolutionHBSSHippocampal NeuronsHippocampusHNHydroxymethylglutaryl Coenzyme A Reductase KinaseHydroxymethylglutaryl Coenzyme A Reductase Kinase Alpha1Hydroxymethylglutaryl Coenzyme A Reductase Kinase Alpha2Hydroxymethylglutaryl Coenzyme A Reductase Kinase Beta1Hydroxymethylglutaryl Coenzyme A Reductase Kinase Beta2Hydroxymethylglutaryl Coenzyme A Reductase Kinase Gamma1Hydroxymethylglutaryl Coenzyme A Reductase Kinase Gamma2AHydroxymethylglutaryl Coenzyme A Reductase Kinase Gamma2BHypoglycemic AgentsKnockoutKOLiver Kinase B1LKB1MaleMammalian Target of RapamycinMap2MetabolismMiceMice, Inbred C57BLMice, TransgenicMicrotubule-Associated Protein 2MTORNerve Cell DifferentiationNerve Fiber GrowthNeuritesNeuroanatomyNeuronNeuronsNon-HumanP13KP70 Ribosomal Protein S6 KinaseP70S6KPhosphatidyl Inositol 3-KinasePolyvinylidene DifluoridePriority JournalProtein ExpressionProtein Kinase BPVDFRatReverse Transcriptase-Polymerase Chain ReactionRT-PCRSDSSignal TransductionSodium Dodecyl SulfateTBSTTris-Buffered Saline+Tween 20Unclassified DrugWild TypeWT
ISSN
0306-4522
Abstract
AMP-activated protein kinase (AMPK) is a serine/threonine kinase that functions as a cellular and whole organism energy sensor to regulate ATP-consuming (anabolic) and ATP-generating (catabolic) pathways. The heterotrimeric AMPK complex consists of a catalytic α-subunit, regulatory β-subunit, and an AMP/ATP-binding γ-subunit. Several alternate isoforms exist for each subunit (α1, α2, β1, β2, γ1, γ2 and γ3). However, little is known of the expression pattern or function of the individual catalytic complexes in regulating neuronal structure. In this study, we examined the role of AMPK subunits in differentiating hippocampal neurons. We found that during development, the expression of AMPK subunits increase and that activation of AMPK by energetic stress inhibits neuronal development at multiple stages, not only during axon outgrowth, but also during dendrite growth and arborization. The presence of a single functional AMPK catalytic complex was sufficient to mediate these inhibitory effects of energetic stress. Activation of AMPK mediates these effects by suppressing both the mTOR and Akt signaling pathways. These findings demonstrate that the energy-sensing AMPK pathway regulates neuronal structure in distinct regions of developing neurons at multiple stages of development. © 2013 IBRO.
URI
http://hdl.handle.net/20.500.11750/2669
DOI
10.1016/j.neuroscience.2013.11.048
Publisher
Elsevier Ltd
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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