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Extracts from Dendropanax morbifera Leaves Have Modulatory Effects on Neuroinflammation in Microglia

Title
Extracts from Dendropanax morbifera Leaves Have Modulatory Effects on Neuroinflammation in Microglia
Authors
Shim, HJ[Shim, Hyun-Jung]Park, S[Park, Sinwoo]Lee, JW[Lee, Ji-Won]Park, HJ[Park, Hye-Jin]Baek, SH[Baek, Seung-Hoon]Kim, EK[Kim, Eun-Kyoung]Yu, SW[Yu, Seong-Woon]
DGIST Authors
Shim, HJ[Shim, Hyun-Jung]; Park, S[Park, Sinwoo]; Lee, JW[Lee, Ji-Won]; Kim, EK[Kim, Eun-Kyoung]Yu, SW[Yu, Seong-Woon]
Issue Date
2016-02
Citation
American Journal of Chinese Medicine, 44(1), 119-132
Type
Article
Article Type
Article
Keywords
Dendropanax MorbiferaM2 PolarizationMicrogliaNeuroinflammationNF-Kappa B
ISSN
0192-415X
Abstract
Dendropanax morbifera (D. morbifera), a species endemic to Korea, is largely distributed throughout the southern part of the country. Its leaves, stems, roots, and seeds have been used as a form of alternative medicine for various diseases and neurological disorders including paralysis, stroke, and migraine. However, the molecular mechanisms that underlie the remedial effects of D. morbifera remain largely unknown. In this paper, extracts from D. morbifera leaves were prepared using ethyl acetate as a solvent (abbreviated as DMLE). The modulatory effects of DMLE on neuroinflammation were studied in a lipopolysaccharide (LPS)-stimulated BV2 murine microglial cell line. Production of pro-inflammatory cytokines, activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-κB), and different M1/M2 activation states of microglia were examined. DMLE treatment suppressed the production of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nitric oxide (NO) in LPS-stimulated BV2 cells. DMLE treatment also attenuated the activation of MAPKs and NF-κB. In a novel discovery, we found that DMLE up-regulated the marker genes representing an alternative, anti-inflammatory M2 polarization, while suppressing the expression of the classical, pro-inflammatory M1 activation state genes. Here, we uncovered the cellular mechanisms underlying the beneficial effects of D. morbifera against neuroinflammation using BV2 microglia cells. These results strongly suggest that DMLE was able to counter the effects of LPS on BV2 cells via control of microglia polarization states. Additionally, study results indicated that DMLE may have therapeutic potential as a neuroinflammation-suppressing treatment for neurodegenerative diseases. © 2016 World Scientific Publishing Company.
URI
http://hdl.handle.net/20.500.11750/2728
DOI
10.1142/S0192415X16500087
Publisher
World Scientific Publishing Co. Pte Ltd
Related Researcher
Files:
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Collection:
Brain and Cognitive SciencesLab of Neuro-Metabolism & Neurometabolomic Research Center1. Journal Articles


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