Cited 2 time in webofscience Cited 5 time in scopus

From a meso- to micro-scale connectome: array tomography and mGRASP

Title
From a meso- to micro-scale connectome: array tomography and mGRASP
Authors
Rah, JC[Rah, Jong-Cheol]Feng, LQ[Feng, Linqing]Druckmann, S[Druckmann, Shaul]Lee, H[Lee, Hojin]Kim, J[Kim, Jinhyun]
DGIST Authors
Rah, JC[Rah, Jong-Cheol]
Issue Date
2015-06-04
Citation
Frontiers in Neuroanatomy, 9
Type
Article
Article Type
Review
Keywords
3D AtlasingArray TomographyBrain MappingBrain TomographyConnectomeFunctional AssessmentGFP Reconstitution Across Synaptic PartnerHumanMGRASPMultiple ScalesNerve Cell NetworkNon-HumanPhotostimulationProtein ExpressionProteomicsReviewSpatial Analysis
ISSN
1662-5129
Abstract
Mapping mammalian synaptic connectivity has long been an important goal of neuroscience because knowing how neurons and brain areas are connected underpins an understanding of brain function. Meeting this goal requires advanced techniques with single synapse resolution and large-scale capacity, especially at multiple scales tethering the meso- and micro-scale connectome. Among several advanced LM-based connectome technologies, Array Tomography (AT) and mammalian GFP-Reconstitution Across Synaptic Partners (mGRASP) can provide relatively high-throughput mapping synaptic connectivity at multiple scales. AT- and mGRASP-assisted circuit mapping (ATing and mGRASPing), combined with techniques such as retrograde virus, brain clearing techniques, and activity indicators will help unlock the secrets of complex neural circuits. Here, we discuss these useful new tools to enable mapping of brain circuits at multiple scales, some functional implications of spatial synaptic distribution, and future challenges and directions of these endeavors. © 2015 Rah, Feng, Druckmann, Lee and Kim.
URI
http://hdl.handle.net/20.500.11750/2892
DOI
10.3389/fnana.2015.00078
Publisher
Frontiers Research Foundation
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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