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Development of transgenic strawberry plants expressing monellin, a sweet protein

Title
Development of transgenic strawberry plants expressing monellin, a sweet protein
Translated Title
Development of transgenic strawberry plants expressing monellin, a sweet protein
Authors
Min, S.R.[Min, Sung Ran]Ko, S.M.[Ko, Suk Min]Lyu, J.I.[Lyu, Jae Il]Park, J.H.[Park, Ji Hyun]Yi, S.Y.[Yi, So Young]Lee, I.-H.[Lee, In Ha]Kim, H.S.[Kim, Hyun Sook]Kim, T.I.[Kim, Tae Il]Choi, P.S.[Choi, Pil Son]Jeong, W.-J.[Jeong, Won Joong]Kim, S.W.[Kim, Suk Weon]Kim, J.[Kim, Jong Hyun]Liu, J.R.[Liu, Jang Ryol]
DGIST Authors
Liu, J.R.[Liu, Jang Ryol]
Issue Date
2015
Citation
Journal of Plant Biotechnology, 42(3), 180-185
Type
Article
Article Type
Article
Keywords
Agrobacterium-Mediated TransformationMonellinStrawberrySweet Protein
ISSN
1229-2818
Abstract
Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants. © Korean Society for Plant Biotechnology.
URI
http://hdl.handle.net/20.500.11750/2957
DOI
10.5010/JPB.2015.42.3.180
Publisher
Korean Society of Plant Biotechnology
Files:
There are no files associated with this item.
Collection:
School of Undergraduate Studies1. Journal Articles


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