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Planar Hall magnetoresistive aptasensor for thrombin detection
- Planar Hall magnetoresistive aptasensor for thrombin detection
- Sinha, B[Sinha, B.]; Ramulu, TS[Ramulu, T. S.]; Kim, KW[Kim, K. W.]; Venu, R[Venu, R.]; Lee, JJ[Lee, J. J.]; Kim, CG[Kim, C. G.]
- DGIST Authors
- Ramulu, TS[Ramulu, T. S.]; Kim, KW[Kim, K. W.]; Venu, R[Venu, R.]; Kim, CG[Kim, C. G.]
- Issue Date
- Biosensors and Bioelectronics, 59, 140-144
- Article Type
- Aptamer; Aptamers, Nucleotide; Aptasensor; Biosensing Techniques; Biosensor; Chemistry; Clinical Diagnosis; Clinical Diagnostics; Colorimetry; Concentration (Parameters); Devices; Diagnosis; Dimeticone; DNA; Electric Potential; Electrochemistry; Enzyme Assay; Enzymes; Equipment Design; Evaluation Study; Fluorescence; Genetic Procedures; Gold Deposits; High Signal-to-Noise Ratio; Human; Humans; Limit of Detection; Lower Detection Limit; Magnetic Nano-Particle; Magnetite Nanoparticle; Magnetite Nanoparticles; Magneto Resistive Sensors; Morphology; Planar Hall Magnetoresistive Aptasensor; Planar Hall Magnetoresistive Sensor; Protein Analysis; Proteins; Quartz Crystal Microbalance; Sandwich; Scanning Electron Microscopy; Sensors; Signal-to-Noise Ratio; Signal Noise Ratio; Silicones; Streptavidin; Superparamagnetics; Surface Plasmon Resonance; Thrombin; Thrombin Aptamer
- The use of aptamer-based assays is an emerging and attractive approach in disease research and clinical diagnostics. A sensitive aptamer-based sandwich-type sensor is presented to detect human thrombin using a planar Hall magnetoresistive (PHR) sensor in cooperation with superparamagnetic labels. A PHR sensor has the great advantages of a high signal-to-noise ratio, a small offset voltage and linear response in the low-field region, allowing it to act as a high-resolution biosensor. In the system presented here, the sensor has an active area of 50. μm×50. μm with a 10-nm gold layer deposited onto the sensor surface prior to the binding of thiolated DNA primary aptamer. A polydimethylsiloxane well of 600-μm radius and 1-mm height was prepared around the sensor surface to maintain the same specific area and volume for each sensor. The sensor response was traced in real time upon the addition of streptavidin-functionalized magnetic labels on the sensor. A linear response to the thrombin concentration in the range of 86. pM-8.6. μM and a lower detection limit down to 86. pM was achieved by the proposed present method with a sample volume consumption of 2. μl. The proposed aptasensor has a strong potential for application in clinical diagnosis. © 2014 Elsevier B.V.
- Elsevier Ltd
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