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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by gamma-irradiation (Co-60) of adventitious roots
- Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by gamma-irradiation (Co-60) of adventitious roots
- Zhang, JY[Zhang, Jun-Ying]; Sun, HJ[Sun, Hyeon-Jin]; Song, IJ[Song, In-Ja]; Bae, TW[Bae, Tae-Woong]; Kang, HG[Kang, Hong-Gyu]; Ko, SM[Ko, Suk-Min]; Kwon, YI[Kwon, Yong-Ik]; Kim, IW[Kim, Il-Woung]; Lee, J[Lee, Jaechun]; Park, SY[Park, Shin-Young]; Lim, PO[Lim, Pyung-Ok]; Kim, YH[Kim, Yong Hwan]; Lee, HY[Lee, Hyo-Yeon]
- DGIST Authors
- Lim, PO[Lim, Pyung-Ok]
- Issue Date
- Journal of Ginseng Research, 38(3), 220-225
- Article Type
- 2,4 Dichlorophenoxyacetic Acid; Adventitious Root; Callus (Plant); Cell Proliferation; Cobalt 60; Embryo Development; Explant; Gamma Irradiation; Germination; Gibberellic Acid; Ginseng; Kinetin; Mutant; Mutant Adventitious Roots; Non-Human; Panax Ginseng Meyer; Plant Regeneration; Priority Journal; Shoot; Soil; Somatic Embryo; Somatic Embryogenesis; Wild Type
- An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. © 2014, The Korean Society of Ginseng, Published by Elsevier. All rights reserved.
- Korean Society of Ginseng
- Related Researcher
Lim, Pyung Ok
CBRG(Complex Biology Research Group)
Aging and programmen cell death in plants; RNA-based regulation of leaf aging; Synthetic genetics-based aging reprogramming
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- Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
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