Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jeong, Jin-Woo | - |
dc.contributor.author | Lee, Hye Hyeon | - |
dc.contributor.author | Han, Min Ho | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.contributor.author | Hong, Su Hyun | - |
dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.available | 2017-07-11T06:22:37Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2014-03 | - |
dc.identifier.issn | 1472-6882 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/3107 | - |
dc.description.abstract | Background: Poria cocos Wolf, a medicinal fungus, is widely used in traditional medicines in East Asian countries owing to its various therapeutic potentials. Although several studies have demonstrated the anti-inflammatory activity of this fungus, its underlying mechanisms have not yet been clearly defined.Methods: In the present study, we have demonstrated the anti-inflammatory effects of ethanol extract of P. cocos (EEPC) in lipopolysaccaride (LPS)-stimulated RAW 264.7 macrophages. As inflammatory parameters, the productions of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-1β and tumor necrosis factor (TNF)-α were evaluated. We also examined the EEPC's effect on the nuclear factor-kappaB (NF-κB) signaling pathway.Results: Our results indicated that EEPC exhibits a potent inhibitory effect on NO production and inhibits PGE2 release in LPS-induced macrophages without affecting cell viability. EEPC also significantly attenuated LPS-induced secretion of inflammatory cytokines IL-1β and TNF-α. Additionally, LPS-induced expression of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, IL-1β, and TNF-α was decreased by pre-treatment with EEPC at the transcriptional level. Moreover, EEPC clearly inhibited LPS-induced nuclear translocation of NF-κB p65 subunits, which correlated with EEPC's inhibitory effects on inhibitor kappaB (IκB) degradation. Moreover, EEPC clearly suppressed the LPS-induced DNA-binding activity of NF-κB, as well as the nuclear translocation of the NF-κB p65, which correlated with EEPC's inhibitory effects on inhibitor kappaB (IκB) degradation.Conclusions: Taken together, our data indicates that EEPC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2, IL-1β, and TNF-α through inactivation of the NF-κB signaling pathway, supporting the pharmacological basis of P. cocos as a traditional herbal medicine for treatment of inflammation and its associated disorders. © 2014 Jeong et al.; licensee BioMed Central Ltd. | - |
dc.language | English | - |
dc.publisher | BioMed Central Ltd. | - |
dc.title | Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/1472-6882-14-101 | - |
dc.identifier.scopusid | 2-s2.0-84899135480 | - |
dc.identifier.bibliographicCitation | BMC Complementary and Alternative Medicine, v.14 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordAuthor | Poria cocos | - |
dc.subject.keywordAuthor | RAW 264.7 cells | - |
dc.subject.keywordAuthor | Anti-inflammation | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | ANTIINFLAMMATORY ACTIVITY | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITIES | - |
dc.subject.keywordPlus | PROSTAGLANDIN E-2 | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | TRITERPENES | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | DERMATITIS | - |
dc.citation.title | BMC Complementary and Alternative Medicine | - |
dc.citation.volume | 14 | - |