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Construction and Application of Elastin Like Polypeptide Containing IL-4 Receptor Targeting Peptide

Title
Construction and Application of Elastin Like Polypeptide Containing IL-4 Receptor Targeting Peptide
Authors
Sarangthem, V[Sarangthem, Vijaya]Cho, EA[Cho, Eun A.]Bae, SM[Bae, Sang Mun]Singh, TD[Singh, Thoudam Debraj]Kim, SJ[Kim, Sun-Ji]Kim, S[Kim, Soyoun]Jeon, WB[Jeon, Won Bae]Lee, BH[Lee, Byung-Heon]Park, RW[Park, Rang-Woon]
DGIST Authors
Jeon, WB[Jeon, Won Bae]
Issue Date
2013-12-10
Citation
PLoS ONE, 8(12)
Type
Article
Article Type
Article
Keywords
Animal ExperimentAnimal ModelAnimal TissueAnimalsAntineoplastic ActivityCancer CellCancer TissueCell HomingCell Line, TumorControlled StudyDrug Delivery SystemsDrug FormulationDrug Receptor BindingDrug SpecificityDrug TargetingElastinElastin-Like Polypeptide PolymerFemaleHumanHuman CellHumansIn Vitro StudyInterleukin-4 ReceptorInterleukin-4 Receptor Alpha SubunitLung CancerMiceMice, Inbred BALB CMice, NudeMouseNeoplasm ProteinsNeoplasmsNon-HumanPeptidesPolymerReceptor AffinitySurface Plasmon ResonanceTranscription Factor AP-1Unclassified DrugXenograft Model Antitumor Assays
ISSN
1932-6203
Abstract
Various human solid tumors highly express IL-4 receptors which amplify the expression of some of anti-apoptotic proteins, preventing drug-induced cancer cell death. Thus, IL-4 receptor targeted drug delivery can possibly increase the therapeutic efficacy in cancer treatment. Macromolecular carriers with multivalent targeting moieties offered great advantages in cancer therapy as they not only increase the plasma half-life of the drug but also allow delivery of therapeutic drugs to the cancer cells with higher specificity, minimizing the deleterious effects of the drug on normal cells. In this study we designed a library of elastin like polypeptide (ELP) polymers containing tumor targeting AP1 peptide using recursive directional ligation method. AP1 was previously discovered as an atherosclerotic plaque and breast tumor tissue homing peptide using phage display screening method, and it can selectively bind to the interleukin 4 receptor (IL-4R). The fluorescently labeled [AP1-V 12]6, an ELP polymer containing six AP1 enhanced tumor-specific targeting ability and uptake efficiency in H226 and MDA-MB-231 cancer cell lines in vitro. Surface plasmon resonance analysis showed that multivalent presentation of the targeting ligand in the ELP polymer increased the binding affinity towards IL-4 receptor compared to free peptide. The binding of [AP1-V12]6 to cancer cells was remarkably reduced when IL-4 receptors were blocked by antibody against IL-4 receptor further confirmed its binding. Importantly, the Cy5.5-labeled [AP1-V12]6 demonstrated excellent homing and longer retention in tumor tissues in MDA-MB-231 xenograft mouse model. Immunohistological studies of tumor tissues further validated the targeting efficiency of [AP1-V12]6 to tumor tissue. These results indicate that designed [AP1-V12] 6 can serve as a novel carrier for selective delivery of therapeutic drugs to tumors. © 2013 Sarangthem et al.
URI
http://hdl.handle.net/20.500.11750/3168
DOI
10.1371/journal.pone.0081891
Publisher
Public Library of Science
Related Researcher
Files:
There are no files associated with this item.
Collection:
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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