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Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide

Title
Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide
Authors
Choi, SK[Choi, Seong-Kyoon]Park, JK[Park, Jin-Kyu]Lee, KM[Lee, Kyeong-Min]Lee, SK[Lee, Soo-Keun]Jeon, WB[Jeon, Won Bae]
DGIST Authors
Choi, SK[Choi, Seong-Kyoon]; Park, JK[Park, Jin-Kyu]; Lee, KM[Lee, Kyeong-Min]Lee, SK[Lee, Soo-Keun]Jeon, WB[Jeon, Won Bae]
Issue Date
2013-11
Citation
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 101(8), 1329-1339
Type
Article
Article Type
Article
Keywords
2&apos,3&aposCyclic Nucleotide 3&aposPhosphodiesteraseAmino Acid MotifsAstrocyteAstrocytesBeta TubulinBiological MarkerBiological MarkersBiological MaterialsCell AdhesionCell DifferentiationCell LineageCell ProliferationChemical StructureChemistryComplementary DnaConcentration-Dependent MannersCopolymersCytologyElastinElastin-Like PolypeptideElastin-Like PolypeptidesEscherichia ColiExtracellular MatricesExtracellular MatrixFourier TransformationGene ExpressionGene SequenceGlial Fibrillary Acidic ProteinGlyceraldehyde 3 Phosphate DehydrogenaseHumanHuman CellHumansInfrared SpectroscopyMessenger RNAMetabolismMicrotubule Associated Protein 2MorphologyNeural Progenitor CellsNeural Stem CellNeural Stem CellsNeural Tissue EngineeringNeuron Specific EnolaseNucleotide SequencePeptidePeptidesPhase TransitionPLGA ScaffoldsPoly-Lactide-Co-GlycolidePolyglactinPolyglactin 910PolypeptidePorosityProceduresProtein MotifReal Time Polymerase Chain ReactionRetinoic AcidReverse Transcriptase-Polymerase Chain ReactionScaffolds (Biology)Scanning Electron MicroscopySpectroscopy, Fourier Transform InfraredStem CellsStem/Progenitor CellsSurface ModificationSurface PropertySurface TreatmentTissue EngineeringTissue Engineering ApplicationsTissue ScaffoldTissue ScaffoldsTretinoinUnclassified Drug
ISSN
1552-4973
Abstract
Poly(lactide-co-glycolide) (PLGA) and elastin-like polypeptide (ELP) have been widely used as a biodegradable scaffold and thermoresponsive matrix, respectively. However, little attention has focused on the combinatorial use of these biomaterials for tissue engineering applications. An ELP matrix TGPG[VGRGD(VGVPG)6]20WPC (referred to as REP) contains multiple Arg-Gly-Asp motifs. This study fabricated porous PLGA scaffolds coated with various concentration of matrix via thermally induced phase transition to improve adhesion-mediated proliferation and differentiation of neural progenitor cells. Matrix-coated scaffolds were characterized by FTIR, SEM, and hematoxylin and eosin staining with respect to coating efficiency, porosity, and pore size and shape. On the matrix-coated scaffolds, cells grew as a single cell or associated each other to form a multicellular layer or cluster. In biological evaluations, cell adhesion and proliferation were significantly promoted in a matrix concentration-dependent manner. More importantly, in combination with retinoic acid, the differentiation of progenitor cells into neuronal and astroglial lineages was highly stimulated in the cells cultured on matrix-coated scaffolds than on untreated controls. Taken together, our results indicated that the REP matrix-functionalized PLGA scaffolds are suitable for improving neuronal cell functions, and thus applicable for neural tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1329-1339, 2013. Copyright © 2013 Wiley Periodicals, Inc.
URI
http://hdl.handle.net/20.500.11750/3188
DOI
10.1002/jbm.b.32950
Publisher
Wiley Blackwell
Related Researcher
Files:
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Collection:
ETC1. Journal Articles


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