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Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers

Title
Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers
Authors
Kim, YR[Kim, Yang-Rae]Seo, HJ[Seo, Hyo-Ju]Oh, JW[Oh, Jeong-Wook]Lim, H[Lim, Hyunchang]Kim, TH[Kim, Tae Hyun]Kim, H[Kim, Hasuck]
DGIST Authors
Kim, H[Kim, Hasuck]
Issue Date
2013-04
Citation
Electroanalysis, 25(4), 1056-1063
Type
Article
Article Type
Article
Keywords
Electrogenerated ChemiluminescenceImmunoassayImmunoglobulin GQuartz Crystal MicrobalanceSelf-Assembled MonolayersSilica Nanoparticles
ISSN
1040-0397
Abstract
An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy)3 2+-doped silica nanoparticles and calix[4]crown-5 self-assembled monolayers (SAMs). Ru(bpy)3 2+-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30μgmL-1. The detection limit of IgG is 1.5μgmL-1. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
URI
http://hdl.handle.net/20.500.11750/3249
DOI
10.1002/elan.201200548
Publisher
Wiley-VCH Verlag
Files:
There are no files associated with this item.
Collection:
Energy Science and EngineeringETC1. Journal Articles


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