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Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calixcrown-5 Self-Assembled Monolayers
- Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calixcrown-5 Self-Assembled Monolayers
- Kim, YR[Kim, Yang-Rae]; Seo, HJ[Seo, Hyo-Ju]; Oh, JW[Oh, Jeong-Wook]; Lim, H[Lim, Hyunchang]; Kim, TH[Kim, Tae Hyun]; Kim, H[Kim, Hasuck]
- DGIST Authors
- Kim, H[Kim, Hasuck]
- Issue Date
- Electroanalysis, 25(4), 1056-1063
- Article Type
- Electrogenerated Chemiluminescence; Immunoassay; Immunoglobulin G; Quartz Crystal Microbalance; Self-Assembled Monolayers; Silica Nanoparticles
- An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy)3 2+-doped silica nanoparticles and calixcrown-5 self-assembled monolayers (SAMs). Ru(bpy)3 2+-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calixcrown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30μgmL-1. The detection limit of IgG is 1.5μgmL-1. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Wiley-VCH Verlag
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