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Differential cell death and Bcl-2 expression in the mouse retina after glutathione decrease by systemic D,L-buthionine sulphoximine administration

Title
Differential cell death and Bcl-2 expression in the mouse retina after glutathione decrease by systemic D,L-buthionine sulphoximine administration
Authors
Park, MH[Park, Myoung Hee]Kim, SY[Kim, So Yeun]Moon, C[Moon, Chanil]Bae, YC[Bae, Young Chul]Moon, JI[Moon, Jung-Il]Moon, C[Moon, Cheil]
DGIST Authors
Kim, SY[Kim, So Yeun]; Moon, C[Moon, Cheil]
Issue Date
2013-03
Citation
Molecules and Cells, 35(3), 235-242
Type
Article
Article Type
Article
Keywords
Animal CellAnimal ExperimentAnimal ModelAnimal TissueAnimalsAntioxidantsApoptosisBcl-2BSOButhionine SulfoximineCell DeathCell SurvivalControlled StudyGene ExpressionGlaucomaGlutamate-Cysteine LigaseGlutathioneImmunoblottingImmunohistochemistryIn Vivo CultureMaleMammaliaMiceMice, Inbred C57BLMouseNon-HumanOrgan SpecificityOxidative StressProtein BCL 2Protein ExpressionProto-Oncogene Proteins C-BCL-2RetinaRetina CellRetina DegenerationRetinopathy
ISSN
1016-8478
Abstract
Glutathione (GSH) plays a critical role in cellular defense against unregulated oxidative stress in mammalian cells including neurons. We previously demonstrated that GSH decrease using [D, L]-buthionine sulphoximine (BSO) induces retinal cell death, but the underlying mechanisms of this are still unclear. Here, we demonstrated that retinal GSH level is closely related to retinal cell death as well as expression of an anti-apoptotic molecule, Bcl-2, in the retina. We induced differential expression of retinal GSH by single and multiple administrations of BSO, and examined retinal GSH levels and retinal cell death in vivo. Single BSO administration showed a transient decrease in the retinal GSH level, whereas multiple BSO administration showed a persistent decrease in the retinal GSH level. Retinal cell death also showed similar patterns: transient increases of retinal cell death were observed after single BSO administration, whereas persistent increases of retinal cell death were observed after multiple BSO administration. Changes in the retinal GSH level affected Bcl-2 expression in the retina. Immunoblot and immunohistochemical analyses showed that single and multiple administration of BSO induced differential expressions of Bcl-2 in the retina. Taken together, the results of our study suggest that the retinal GSH is important for the survival of retinal cells, and retinal GSH appears to be deeply related to Bcl-2 expression in the retina. Thus, alteration of Bcl-2 expression may provide a therapeutic tool for retinal degenerative diseases caused by retinal oxidative stress such as glaucoma or retinopathy. © 2013 The Korean Society for Molecular and Cellular Biology and Springer Netherlands.
URI
http://hdl.handle.net/20.500.11750/3260
DOI
10.1007/s10059-013-2276-y
Publisher
Korean Society for Molecular and Cellular Biology
Related Researcher
Files:
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Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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