Cited 3 time in webofscience Cited 4 time in scopus

Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices

Title
Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices
Authors
Kim, H[Kim, Hwajeong]Kim, SY[Kim, So Yeun]Nam, S[Nam, Sungho]Ronnett, GV[Ronnett, Gabriele V.]Han, HS[Han, Hyung Soo]Moon, C[Moon, Cheil]Kim, Y[Kim, Youngkyoo]
DGIST Authors
Kim, SY[Kim, So Yeun]; Ronnett, GV[Ronnett, Gabriele V.]; Moon, C[Moon, Cheil]
Issue Date
2012
Citation
Analyst, 137(9), 2047-2053
Type
Article
Article Type
Article
Keywords
AnimalAnimalsCell CountCell Culture TechniquesCell DifferentiationChemistryCulture TechniqueCytologyDrug EffectElectric ConductivityElectrodeElectrodesElectrophysiological ProcessesElectrophysiologyExtracellular SpaceIndium Tin OxideInstrumentationKineticsMetabolismOdorOdorsOlfactory ReceptorOlfactory Receptor NeuronsPotassium ChlorideRatRatsTin CompoundsTin Derivative
ISSN
0003-2654
Abstract
An artificial nose was developed to mimic aspects of sensory transduction of the peripheral mammalian olfactory system. We directly cultured and differentiated rat olfactory sensory neurons (OSNs) on indium-tin oxide electrodes of planar triode substrates without a coupling agent. Direct voltage (∼50 μV) and current (∼250 nA) signals were measured simultaneously when OSNs on the planar triode substrates were exposed to odorant mixtures. The response signals were sensitive to the concentration of the odorant mixture, with a typical lifetime, shape, and adaptation profile as seen in responses upon repeated stimulation in vivo. We found that the rising time to the peak current was ∼161 ms, while the signal back to baseline was in 1.8 s, which are in agreement with the natural intracellular electrophysiological responses. These results provide the first evidence that mature OSNs grown in a planar triode device are able to detect direct electrophysiological responses to odorants. © 2012 The Royal Society of Chemistry.
URI
http://hdl.handle.net/20.500.11750/3422
DOI
10.1039/c2an16205a
Publisher
Royal Society of Chemistry
Related Researcher
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles
Brain and Cognitive SciencesETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE