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Developmentally regulated GTP-binding protein 2 depletion leads to mitochondrial dysfunction through downregulation of dynamin-related protein 1

Title
Developmentally regulated GTP-binding protein 2 depletion leads to mitochondrial dysfunction through downregulation of dynamin-related protein 1
Authors
Vo, Mai-TramKo, Myoung SeokLee, Unn HwaYoon, Eun HyeLee, Byung JuCho, Wha JaHa, Chang ManKim, KyungjinPark, Jeong Woo
Issue Date
2017-05-13
Citation
Biochemical and Biophysical Research Communications, 486(4), 1014-1020
Type
Article
Article Type
Article
Keywords
ApoptosisApoptosis
ISSN
0006-291X
Abstract
Mitochondrial dynamics, including constant fusion and fission, play critical roles in maintaining mitochondrial morphology and function. Here, we report that developmentally regulated GTP-binding protein 2 (DRG2) regulates mitochondrial morphology by modulating the expression of the mitochondrial fission gene dynamin-related protein 1 (Drp1). shRNA-mediated silencing of DRG2 induced mitochondrial swelling, whereas expression of an shRNA-resistant version of DRG2 decreased mitochondrial swelling in DRG2-depleted cells. Analysis of the expression levels of genes involved in mitochondrial fusion and fission revealed that DRG2 depletion significantly decreased the level of Drp1. Overexpression of Drp1 rescued the defect in mitochondrial morphology induced by DRG2 depletion. DRG2 depletion reduced the mitochondrial membrane potential, oxygen consumption rate (OCR), and amount of mitochondrial DNA (mtDNA), whereas it increased reactive oxygen species (ROS) production and apoptosis. Taken together, our data demonstrate that DRG2 acts as a regulator of mitochondrial fission by controlling the expression of Drp1. © 2017 Elsevier Inc.
URI
http://hdl.handle.net/20.500.11750/4177
DOI
10.1016/j.bbrc.2017.03.154
Publisher
Elsevier B.V.
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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