Cited 0 time in webofscience Cited 0 time in scopus

Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition

Title
Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
Authors
Huang, YunKim, Jong KyoungDang Vinh DoLee, CarolinePenfold, Christopher A.Zylicz, Jan J.Marioni, John C.Hackett, Jamie A.Surani, M. Azim
DGIST Authors
Kim, Jong Kyoung
Issue Date
2017-03-21
Citation
eLife, 6
Type
Article
Article Type
Article
Keywords
Differential ExpressionDNA MethylationEarly Mouse DevelopmentGene ExpressionHost GenesHuman Preimplantation EmbryosRNA Seq ExperimentsSingle CellStem CellsTransposable Elements
ISSN
2050-084X
Abstract
The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional misregulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression. © Huang et al.
URI
http://hdl.handle.net/20.500.11750/4214
DOI
10.7554/eLife.22345
Publisher
eLife Sciences Publications Ltd
Related Researcher
Files:
There are no files associated with this item.
Collection:
New BiologyETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE