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Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
- Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
- Huang, Yun; Kim, Jong Kyoung; Dang Vinh Do; Lee, Caroline; Penfold, Christopher A.; Zylicz, Jan J.; Marioni, John C.; Hackett, Jamie A.; Surani, M. Azim
- DGIST Authors
- Kim, Jong Kyoung
- Issue Date
- eLife, 6
- Article Type
- Differential Expression; DNA Methylation; Early Mouse Development; Gene Expression; Host Genes; Human Preimplantation Embryos; RNA Seq Experiments; Single Cell; Stem Cells; Transposable Elements
- The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional misregulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression. © Huang et al.
- eLife Sciences Publications Ltd
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