Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Hong, Bong-Ki | - |
dc.contributor.author | You, Sungyong | - |
dc.contributor.author | Yoo, Seung-Ah | - |
dc.contributor.author | Park, Dohyun | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Cho, Chul-Soo | - |
dc.contributor.author | Kim, Wan-Uk | - |
dc.date.available | 2017-09-11T09:28:25Z | - |
dc.date.created | 2017-09-11 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/4453 | - |
dc.description.abstract | Fibroblast-like synoviocytes (FLSs) constitute a major cell subset of rheumatoid arthritis (RA) synovia. Dysregulation of microRNAs (miRNAs) has been implicated in activation and proliferation of RA-FLSs. However, the functional association of various miRNAs with their targets that are characteristic of the RA-FLS phenotype has not been globally elucidated. In this study, we performed microarray analyses of miRNAs and mRNAs in RA-FLSs and osteoarthritis FLSs (OA-FLSs), simultaneously, to validate how dysregulated miRNAs may be associated with the RA-FLS phenotype. Global miRNA profiling revealed that miR-143 and miR-145 were differentially upregulated in RA-FLSs compared to OA-FLSs. miR-143 and miR-145 were highly expressed in independent RA-FLSs. The miRNA-target prediction and network model of the predicted targets identified insulin-like growth factor binding protein 5 (IGFBP5) and semaphorin 3A (SEMA3A) as potential target genes downregulated by miR-143 and miR-145, respectively. IGFBP5 level was inversely correlated with miR-143 expression, and its deficiency rendered RA-FLSs more sensitive to TNFα stimulation, promoting IL-6 production and NF-κB activity. Moreover, SEMA3A was a direct target of miR-145, as determined by a luciferase reporter assay, antagonizing VEGF"1"6"5-induced increases in the survival, migration and invasion of RA-FLSs. Taken together, our data suggest that enhanced expression of miR-143 and miR-145 renders RA-FLSs susceptible to TNFα and VEGF"1"6"5 stimuli by downregulating IGFBP5 and SEMA3A, respectively, and that these miRNAs could be therapeutic targets. © 2017 KSBMB. | - |
dc.language | English | - |
dc.publisher | 생화학분자생물학회 | - |
dc.title | MicroRNA-143 and-145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/emm.2017.108 | - |
dc.identifier.wosid | 000410049800002 | - |
dc.identifier.scopusid | 2-s2.0-85026863628 | - |
dc.identifier.bibliographicCitation | Experimental and Molecular Medicine, v.49, no.8 | - |
dc.identifier.kciid | ART002251793 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | SYNOVIOCYTES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MIR-143/145 | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | ALPHA | - |
dc.citation.number | 8 | - |
dc.citation.title | Experimental and Molecular Medicine | - |
dc.citation.volume | 49 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology; Medicine, Research & Experimental | - |
dc.type.docType | Article | - |