Cited 0 time in webofscience Cited 0 time in scopus

All-in-one low-intensity pulsed ultrasound stimulation system using piezoelectric micromachined ultrasonic transducer (pMUT) arrays for targeted cell stimulation

Title
All-in-one low-intensity pulsed ultrasound stimulation system using piezoelectric micromachined ultrasonic transducer (pMUT) arrays for targeted cell stimulation
Authors
Lee, Won-JunYoo, Seung-JunJung, Joon-TaekKang, Woo-JinWang, WeiMoon, Che-IlChoi, Hong-Soo
DGIST Authors
Lee, Won-Jun; Yoo, Seung-Jun; Jung, Joon-Taek; Kang, Woo-Jin; Moon, Che-IlChoi, Hong-Soo
Issue Date
2017-12
Citation
Biomedical Microdevices, 19(4)
Type
Article
Article Type
Article
Keywords
BiocompatibilityBrain-FunctionCell CultureCell ProliferationCell StimulationCell Stimulation SystemCellsChondrocytesCulture TemperatureDifferentiationElectrical-StimulationLow-FrequencyLow-Intensity Pulsed UltrasoundNerve Growth-FactorPC12 CellsPiezoelectric Micromachined Ultrasonic Transducer (PMUT)Piezoelectric TransducersPiezoelectricitypMUTPrint Circuit BoardsPrinted Circuit BoardsProliferationRatResonance Frequencies, CytologyTranscranial Magnetic StimulationTransducersUltrasonic TestingUltrasonic TransducerUltrasonic Transducers, Cell Proliferation RateUltrasound
ISSN
1387-2176
Abstract
A novel cell-stimulation system was fabricated using 10 × 29 piezoelectric micromachined ultrasonic transducer (pMUT) arrays for targeted ultrasonic cell stimulation. Both the diameter of a single pMUT element and the edge-to-edge gap were 120 μm, and the size of a pMUT array was 2.27 × 6.84 mm, to be placed at the bottom of a Transwell. The measured resonance frequency of a single pMUT element was 1.48 ± 0.13 MHz and the measured acoustic intensity of the pMUT array was 0.15 ± 0.03 MPa at 1 mm away from the transducer. A pMUT array was mounted on a print circuit board (PCB), which was designed in accordance with the size of a 12-well Transwell. The Transwell was placed on the PCB and wire bonding was performed to electrically connect the PCB and pMUT arrays. After wiring, the PCB and pMUT arrays were coated with 2.6-μm thick parylene-C to ensure biocompatibility and waterproofing. PC12 cells were used for ultrasonic cell stimulation tests to examine the proposed all-in-one low-intensity pulsed ultrasound stimulation system. Various stimulation times and duty cycles were used simultaneously for cell proliferation in a confined cell culture environment. All stimulation groups showed increased cell proliferation rates, in the range 138–166%, versus the proliferation rate of the control group. © 2017, Springer Science+Business Media, LLC.
URI
http://hdl.handle.net/20.500.11750/4582
DOI
10.1007/s10544-017-0228-6
Publisher
Springer New York LLC
Related Researcher
Files:
There are no files associated with this item.
Collection:
Robotics EngineeringBio-Micro Robotics Lab1. Journal Articles
Brain and Cognitive SciencesMoon Lab1. Journal Articles
Robotics EngineeringETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE