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종이기반 농도 구배 형성 칩을 통한 포도당 발색 반응 검사

Title
종이기반 농도 구배 형성 칩을 통한 포도당 발색 반응 검사
Translated Title
A Colorimetric Glucose Assay via Concentration Gradient Paper Chip
Authors
김태훈신현영이윤일태기식김민석
DGIST Authors
김민석
Issue Date
2017-12
Citation
의공학회지, 38(6), 302-307
Type
Article
Keywords
Microfluidic paper-based analytical devices(μPADs)Concentration gradientColorimetric glucose assay
ISSN
1229-0807
Abstract
This paper presents a paper-based concentration gradient chip to analyze colorimetric glucose assay. The paper-based concentration gradient chip was fabricated through a wax patterning technique that can design the fluidic channel by selectively printing hydrophobic and hydrophilic areas. Afterwards, glucose and dilution solutions were loaded into the inlet of a concentration gradient chip and each solution was then mixed sequentially at mixing channel. Finally, concentration gradient was formed at each outlet of the chip. To measure the glucose concentration of the solution in outlets, we conducted colorimetric glucose assay with fixed concentration of glucose solution (0, 5, 10, 15 and 20 mM) and obtained normalized intensity. Subsequently, glucose concentrations of the outlets were calculated by substituting the normalized intensity to linear regression function based on the normalized intensity of fixed glucose concentration. Finally, the concentration gradient of glucose was formed on the chip with the result of colorimetric assay. The concentration gradient paper chip has the potential to accurately analyze unknown glucose concentration.
URI
http://hdl.handle.net/20.500.11750/4863
DOI
10.9718/JBER.2017.38.6.302
Publisher
대한의용생체공학회
Related Researcher
Files:
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Collection:
ETC1. Journal Articles


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