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Antitumorigenic effect of atmospheric-pressure dielectric barrier discharge on human colorectal cancer cells via regulation of Sp1 transcription factor

Title
Antitumorigenic effect of atmospheric-pressure dielectric barrier discharge on human colorectal cancer cells via regulation of Sp1 transcription factor
Authors
Han, DuksunCho, Jin HyoungLee, Ra HamBang, WoongPark, KyunghoKim, Minseok S.Shim, Jung-HyunChae, Jung-IlMoon, Se Youn
DGIST Authors
Kim, Minseok S.
Issue Date
2017-02-22
Citation
Scientific Reports, 7
Type
Article
Article Type
Article
Keywords
ApoptosisCycle ArrestDown RegulationGastric CancerGrowthIn VitroMetastasisNonthermal PlasmaProgressionSuppresses
ISSN
2045-2322
Abstract
Human colorectal cancer cell lines (HT29 and HCT116) were exposed to dielectric barrier discharge (DBD) plasma at atmospheric pressure to investigate the anticancer capacity of the plasma. The dose- and time-dependent effects of DBDP on cell viability, regulation of transcription factor Sp1, cell-cycle analysis, and colony formation were investigated by means of MTS assay, DAPI staining, propidium iodide staining, annexin V-FITC staining, Western blot analysis, RT-PCR analysis, fluorescence microscopy, and anchorage-independent cell transformation assay. By increasing the duration of plasma dose times, significant reductions in the levels of both Sp1 protein and Sp1 mRNA were observed in both cell lines. Also, expression of negative regulators related to the cell cycle (such as p53, p21, and p27) was increased and of the positive regulator cyclin D1 was decreased, indicating that the plasma treatment led to apoptosis and cell-cycle arrest. In addition, the sizes and quantities of colony formation were significantly suppressed even though two cancer promoters, such as TPA and epidermal growth factor, accompanied the plasma treatment. Thus, plasma treatment inhibited cell viability and colony formation by suppressing Sp1, which induced apoptosis and cell-cycle arrest in these two human colorectal cancer cell lines.
URI
http://hdl.handle.net/20.500.11750/5018
DOI
10.1038/srep43081
Publisher
NATURE PUBLISHING GROUP
Related Researcher
Files:
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Collection:
ETC1. Journal Articles


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