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Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum
- Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum
- Kim, Eunjoo; Lee, Se Geun; Kim, Hyun-Chul; Lee, Sung Jun; Baek, Chul Su; Choi, Eun-Sook; Jeong, Sang Won
- DGIST Authors
- Kim, Eunjoo; Lee, Se Geun; Kim, Hyun-Chul; Lee, Sung Jun; Jeong, Sang Won
- Issue Date
- Separation Science and Technology, 48(17), 2600-2607
- Article Type
- Affinity Isolation; Body Fluids; C-Reactive Protein (CRP); C-Reactive Proteins; Magnetic Nano-Particle; Magnetic Nano-Particles; Nanoparticles; Phosphocholine; Protein Purification; Proteins
- In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation. © 2013 Copyright Taylor and Francis Group, LLC.
- Taylor and Francis Ltd.
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