https://scholar.dgist.ac.kr/handle/20.500.11750/15990 2026-04-04T13:37:27Z 2026-04-04T13:37:27Z Kim, Sojeong Kwak, Jeong-Eun Koh, June-Young Lee, Ji Eun Kook, Hye Won Kim, Minchae Chung, Haerim Kim, Yuri Kim, Soo Jeoong Kim, Jin Seok Cheong, June-Won Lee, Min Goo Lee, Hoyoung Park, Su-Hyung Shin, Eui-Cheol Shin, Saeam Yoon, Sun Och Choi, Il-Kyu Lee, Jeong Seok Cho, Hyunsoo https://scholar.dgist.ac.kr/handle/20.500.11750/58692 2026-02-01T10:40:11Z 2025-06-30T15:00:00Z

Title: NKG2D-mediated cytotoxicity of CD4 cytotoxic T cells in multiple myeloma Author(s): Kim, Sojeong; Kwak, Jeong-Eun; Koh, June-Young; Lee, Ji Eun; Kook, Hye Won; Kim, Minchae; Chung, Haerim; Kim, Yuri; Kim, Soo Jeoong; Kim, Jin Seok; Cheong, June-Won; Lee, Min Goo; Lee, Hoyoung; Park, Su-Hyung; Shin, Eui-Cheol; Shin, Saeam; Yoon, Sun Och; Choi, Il-Kyu; Lee, Jeong Seok; Cho, Hyunsoo Abstract: Emerging evidence indicates that CD4+ T cells contribute to antitumor immunity beyond their traditional roles as helpers or regulators. However, the specific subset of CD4+ T cells mediating beneficial outcomes in patients with multiple myeloma remains unclear. Here, we performed single-cell RNA sequencing and T-cell receptor sequencing on CD4+ T cells sorted from the bone marrow of patients across the stages of myeloma progression. We identified several distinct states of CD4+ cytotoxic T lymphocytes (CTLs) that were significantly increased and clonally expanded in patients with myeloma. CD4+ CTLs displayed transcriptional and phenotypic characteristics indicative of cytotoxicity, demonstrating their ability to directly kill myeloma cells. This cytotoxicity, however, was abrogated by NKG2D blockade. Notably, the abundance of NKG2D+CD4+ CTLs correlated with improved survival in patients with myeloma. Our findings suggest that harnessing CD4+ CTLs could lead to novel strategies for enhancing immunotherapy outcomes in multiple myeloma.

2025-06-30T15:00:00Z Alam, Rafiqul Lee, Jung-Hyun Shin, Doyun Choi, Il-Kyu Kim, Sunghwan Lim, Heejin Kim, Min-Sik https://scholar.dgist.ac.kr/handle/20.500.11750/58275 2025-12-27T17:10:12Z 2025-10-31T15:00:00Z

Title: Recent advances in single-cell metabolomics using mass spectrometry: emerging challenges and future perspectives Author(s): Alam, Rafiqul; Lee, Jung-Hyun; Shin, Doyun; Choi, Il-Kyu; Kim, Sunghwan; Lim, Heejin; Kim, Min-Sik Abstract: Cellular heterogeneity plays a pivotal role in organismal physiology, influencing developmental processes, disease progression, and therapeutic responses. Single-cell metabolomics (SCM) emerges as a powerful tool to interrogate the metabolic diversity of individual cells, offering insights into cellular phenotypes beyond genomics, or transcriptomics. Recent advancements in microfluidics, automation, and image analysis have enabled minimally invasive single-cell isolation, while development of innovative mass spectrometry (MS)-based techniques has transformed metabolite detection with their high sensitivity, broad detection range, and molecular specificity. Despite challenges such as the non-amplifiable nature of metabolites and their dynamic concentration ranges like proteins, significant progress has been made in MS platforms, ionization methods, and data analysis strategies. This review highlights the latest innovations in SCM, including nano-electrospray ionization, laser desorption/ionization, and other MS techniques, alongside applications in diverse cell types such as cancer cells, plant cells, neurons, stem cells, and immune cells. Integrating SCM with orthogonal single-cell omics holds promise for systems-level understanding, with potential applications in translational and clinical research. Addressing current limitations in throughput, sensitivity, and data processing will be essential to fully unlock the potential of SCM in answering fundamental and applied biological questions.

2025-10-31T15:00:00Z Noh, Soojeong Ryu, Suyeon Jung, Dokyung Shin, Sanghee Jung, Inseong Kang, Sung-Min Kim, Christine S. Choi, Sung-Jin Cho, Hanchae Schwämmle, Melanie Jeong, Youngtae Bucher, Felicitas Choi, Il-Kyu Lee, Shin Yup Im, Sin-Hyeog Yea, Kyungmoo Baek, Moon-Chang https://scholar.dgist.ac.kr/handle/20.500.11750/57322 2025-07-25T02:42:36Z 2024-11-30T15:00:00Z

Title: IL2-mediated modulation of small extracellular vesicles secretion and PD-L1 expression: a novel perspective for neutralizing immune suppression within cancer cells Author(s): Noh, Soojeong; Ryu, Suyeon; Jung, Dokyung; Shin, Sanghee; Jung, Inseong; Kang, Sung-Min; Kim, Christine S.; Choi, Sung-Jin; Cho, Hanchae; Schwämmle, Melanie; Jeong, Youngtae; Bucher, Felicitas; Choi, Il-Kyu; Lee, Shin Yup; Im, Sin-Hyeog; Yea, Kyungmoo; Baek, Moon-Chang Abstract: [No Abstract Available]

2024-11-30T15:00:00Z Jeong, Seongmin Jang, Nawon Kim, Minchae Choi, Il-Kyu https://scholar.dgist.ac.kr/handle/20.500.11750/46130 2025-07-25T02:39:57Z 2023-01-31T15:00:00Z

Title: CD4+ cytotoxic T cells: an emerging effector arm of anti-tumor immunity Author(s): Jeong, Seongmin; Jang, Nawon; Kim, Minchae; Choi, Il-Kyu Abstract: While CD8+ cytotoxic T cells have long been considered the primary effector in controlling tumors, the involvement of CD4+ “helper” T cells in anti-tumor immunity has been underappreciated. The investigations of intra-tumoral T cells, fueled by the recent advances in genomic technologies, have led to a rethinking of the indirect role of CD4+ T cells that have traditionally been described as a “helper”. Accumulating evidence from preclinical and clinical studies indicates that CD4+ T cells can acquire intrinsic cytotoxic properties and directly kill various types of tumor cells in a major histocompatibility complex class II (MHC-II)-dependent manner, as opposed to the indirect “helper” function, thus underscoring a potentially critical contribution of CD4+ cytotoxic T cells to immune responses against a wide range of tumor types. Here, we discuss the biological properties of anti-tumor CD4+ T cells with cytotoxic capability and highlight the emerging observations suggesting their more significant role in anti-tumor immunity than previously appreciated. © 2023 by the The Korean Society for Biochemistry and Molecular Biology

2023-01-31T15:00:00Z