https://scholar.dgist.ac.kr/handle/20.500.11750/353 2026-04-04T22:00:47Z 2026-04-04T22:00:47Z Noorani, Imran de, la Rosa Jorge Choi, Yoon Ha Strong, Alexander Ponstingl, Hannes Vijayabaskar, M.S. Lee, Ju Sung Lee, Eun Min Richard-Londt, Angela Friedrich, Mathias Furlanetto, Federica Fuente, Rocio Banerjee, Ruby Yang, Fengtang Law, Frances Watts, Colin Rad, Roland Vassiliou, George Kim, Jong Kyoung Santarius, Thomas Brandner, Sebastian Bradley, Allan https://scholar.dgist.ac.kr/handle/20.500.11750/12695 2025-07-25T03:27:14Z 2020-06-30T15:00:00Z

Title: PiggyBac mutagenesis and exome sequencing identify genetic driver landscapes and potential therapeutic targets of EGFR-mutant gliomas Author(s): Noorani, Imran; de, la Rosa Jorge; Choi, Yoon Ha; Strong, Alexander; Ponstingl, Hannes; Vijayabaskar, M.S.; Lee, Ju Sung; Lee, Eun Min; Richard-Londt, Angela; Friedrich, Mathias; Furlanetto, Federica; Fuente, Rocio; Banerjee, Ruby; Yang, Fengtang; Law, Frances; Watts, Colin; Rad, Roland; Vassiliou, George; Kim, Jong Kyoung; Santarius, Thomas; Brandner, Sebastian; Bradley, Allan Abstract: Background: Glioma is the most common intrinsic brain tumor and also occurs in the spinal cord. Activating EGFR mutations are common in IDH1 wild-type gliomas. However, the cooperative partners of EGFR driving gliomagenesis remain poorly understood. Results: We explore EGFR-mutant glioma evolution in conditional mutant mice by whole-exome sequencing, transposon mutagenesis forward genetic screening, and transcriptomics. We show mutant EGFR is sufficient to initiate gliomagenesis in vivo, both in the brain and spinal cord. We identify significantly recurrent somatic alterations in these gliomas including mutant EGFR amplifications and Sub1, Trp53, and Tead2 loss-of-function mutations. Comprehensive functional characterization of 96 gliomas by genome-wide piggyBac insertional mutagenesis in vivo identifies 281 known and novel EGFR-cooperating driver genes, including Cdkn2a, Nf1, Spred1, and Nav3. Transcriptomics confirms transposon-mediated effects on expression of these genes. We validate the clinical relevance of new putative tumor suppressors by showing these are frequently altered in patients' gliomas, with prognostic implications. We discover shared and distinct driver mutations in brain and spinal gliomas and confirm in vivo differential tumor suppressive effects of Pten between these tumors. Functional validation with CRISPR-Cas9-induced mutations in novel genes Tead2, Spred1, and Nav3 demonstrates heightened EGFRvIII-glioma cell proliferation. Chemogenomic analysis of mutated glioma genes reveals potential drug targets, with several investigational drugs showing efficacy in vitro. Conclusion: Our work elucidates functional driver landscapes of EGFR-mutant gliomas, uncovering potential therapeutic strategies, and provides new tools for functional interrogation of gliomagenesis. © 2020 The Author(s).

2020-06-30T15:00:00Z Seo, Eunseok Seong, Myeong Ryun Lee, Ji Woong Lim, Heejin Park, Jiwon Kim, Hyungbin Hwang, Hyundo Lee, Dohoon Kim, Jiho Kim, Gwang Hoon Hwang, Dong Soo Lee, Sang Joon https://scholar.dgist.ac.kr/handle/20.500.11750/12043 2025-07-24T07:33:59Z 2020-04-30T15:00:00Z

Title: Anti-Biofouling Features of Eco-Friendly Oleamide-PDMS Copolymers Author(s): Seo, Eunseok; Seong, Myeong Ryun; Lee, Ji Woong; Lim, Heejin; Park, Jiwon; Kim, Hyungbin; Hwang, Hyundo; Lee, Dohoon; Kim, Jiho; Kim, Gwang Hoon; Hwang, Dong Soo; Lee, Sang Joon Abstract: The biofouling of marine organisms on a surface induces serious economic damage. One of the conventional anti-biofouling strategies is the use of toxic chemicals. In this study, a new eco-friendly oleamide-PDMS copolymer (OPC) is proposed for sustainable anti-biofouling and effective drag reduction. The anti-biofouling characteristics of the OPC are investigated using algal spores and mussels. The proposed OPC is found to inhibit the adhesion of algal spores and mussels. The slippery features of the fabricated OPC surfaces are examined by direct measurement of pressure drops in channel flows. The proposed OPC surface would be utilized in various industrial applications including marine vehicles and biomedical devices. © Copyright © 2020 American Chemical Society.

2020-04-30T15:00:00Z Gwon, Mi‐Gyeong An, Hyun‐Jin Kim, Jung‐Yeon Kim, Woon-Hae Gu, Hyemin Kim, Hyun‐Ju Leem, Jaechan Jung, Hyun Jin Park, Kwan‐Kyu https://scholar.dgist.ac.kr/handle/20.500.11750/11427 2025-07-24T07:33:48Z 2019-12-31T15:00:00Z

Title: Anti-fibrotic effects of synthetic TGF-β1 and Smad oligodeoxynucleotide on kidney fibrosis in vivo and in vitro through inhibition of both epithelial dedifferentiation and endothelial-mesenchymal transitions Author(s): Gwon, Mi‐Gyeong; An, Hyun‐Jin; Kim, Jung‐Yeon; Kim, Woon-Hae; Gu, Hyemin; Kim, Hyun‐Ju; Leem, Jaechan; Jung, Hyun Jin; Park, Kwan‐Kyu Abstract: Kidney fibrosis is a common process of various kidney diseases leading to end-stage renal failure irrespective of etiology. Myofibroblasts are crucial mediators in kidney fibrosis through production of extracellular matrix (ECM), but their origin has not been clearly identified. Many study proposed that epithelial and endothelial cells become myofibroblasts by epithelial dedifferentiation and endothelial-mesenchymal transition (EndoMT). TGF-β1/Smad signaling plays a crucial role in partly epithelial-mensencymal transition (EMT) and EndoMT. Thus, we designed the TGF-β1/Smad oligodeoxynucleotide (ODN), a synthetic short DNA containing complementary sequence for Smad transcription factor and TGF-β1 mRNA. Therefore, this study investigated the anti-fibrotic effect of synthetic TGF-β1/Smad ODN on UUO-induced kidney fibrosis in vivo model and TGF-β1-induced in vitro model. To examine the effect of TGF-β1/Smad ODN, we performed various experiments to evaluate kidney fibrosis. The results showed that UUO induced inflammation, ECM accumulation, epithelial dedifferentiation and EndoMT processes, and tubular atrophy. However, synthetic TGF-β1/Smad ODN significantly suppressed UUO-induced fibrosis. Furthermore, synthetic ODN attenuated TGF-β1-induced epithelial dedifferentiation and EndoMT program via blocking TGF-β1/Smad signaling. In conclusion, this study demonstrated that administration of synthetic TGF-β1/Smad ODN attenuates kidney fibrosis, epithelial dedifferentiation, and EndoMT processes. The findings propose the possibility of synthetic ODN as a new effective therapeutic tool for kidney fibrosis. © 2019 Federation of American Societies for Experimental Biology.

2019-12-31T15:00:00Z Lee, Seung Joon Sim, Tae Seok Shin, Hyun Young Lee, Jeong Min Kim, Min. Young. Sunoo, Joseph Lee, Jeong-Gun Yea, Kyungmoo Kim, Young Zoon Van Noort, D. Park, Soo Kyung Kim, Woon-Hae Park, Kyun Woo Kim, Minseok S. https://scholar.dgist.ac.kr/handle/20.500.11750/10981 2025-07-24T07:32:30Z 2019-09-30T15:00:00Z

Title: Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads Author(s): Lee, Seung Joon; Sim, Tae Seok; Shin, Hyun Young; Lee, Jeong Min; Kim, Min. Young.; Sunoo, Joseph; Lee, Jeong-Gun; Yea, Kyungmoo; Kim, Young Zoon; Van Noort, D.; Park, Soo Kyung; Kim, Woon-Hae; Park, Kyun Woo; Kim, Minseok S. Abstract: Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs. © 2019 Lee et al.

2019-09-30T15:00:00Z