https://scholar.dgist.ac.kr/handle/20.500.11750/1162 2026-04-04T17:06:38Z https://scholar.dgist.ac.kr/handle/20.500.11750/58707 Title: PHARMACEUTICAL COMPOSITION COMPRISING IU1-LINEAGE UBIQUITIN-SPECIFIC PROTEASE 14 INHIBITOR AS ACTIVE INGREDIENT FOR ENHANCING ANTICANCER EFFECT OF PROTAC Author(s): 문성현; 이병훈; Tran Thuy Thi Thanh Abstract: The present invention relates to a pharmaceutical composition for enhancing an anticancer effect and, more specifically, identifies that when administered in combination with PROTAC, a composition comprising an IU1-lineage compound, known to inhibit the deubiquitinase USP14, remarkably enhances the prophylactic or therapeutic effect of PROTAC on cancer. In addition, even when PROTAC is used at a lower dose than conventional doses, the utilization of the pharmaceutical composition of the present invention can effectively treat cancer, thus being free from the problems of toxicity and adverse effects. The pharmaceutical composition overcomes the applicative limitations and problems generated upon the use of PROTAC alone and thus can be widely used in the anticancer therapy field. https://scholar.dgist.ac.kr/handle/20.500.11750/57389 Title: Pharmacological inhibition of USP14 delays proteostasis-associated aging in a proteasome-dependent but foxo-independent manner Author(s): Lim, Jin Ju; Noh, Sujin; Kang, Woojun; Hyun, Bom; Lee, Byung-Hoon; Hyun, Seogang Abstract: Aging is often accompanied by a decline in proteostasis, manifested as an increased propensity for misfolded protein aggregates, which are prevented by protein quality control systems, such as the ubiquitin-proteasome system (UPS) and macroautophagy/autophagy. Although the role of the UPS and autophagy in slowing age-induced proteostasis decline has been elucidated, limited information is available on how these pathways can be activated in a collaborative manner to delay proteostasis-associated aging. Here, we show that activation of the UPS via the pharmacological inhibition of USP14 (ubiquitin specific peptidase 14) using IU1 improves proteostasis and autophagy decline caused by aging or proteostatic stress in Drosophila and human cells. Treatment with IU1 not only alleviated the aggregation of polyubiquitinated proteins in aging Drosophila flight muscles but also extended the fly lifespan with enhanced locomotive activity via simultaneous activation of the UPS and autophagy. Interestingly, the effect of this drug disappeared when proteasomal activity was inhibited, but was evident upon proteostasis disruption by foxo mutation. Overall, our findings shed light on potential strategies to efficiently ameliorate age-associated pathologies associated with perturbed proteostasis. Abbreviations: AAAs: amino acid analogs; foxo: forkhead box, sub-group O; IFMs: indirect flight muscles; UPS: ubiquitin-proteasome system; USP14: ubiquitin specific peptidase 14. © 2024 Informa UK Limited, trading as Taylor & Francis Group. 2024-11-30T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/56656 Title: Structural Dynamics Analysis of USP14 Activation by AKT-Mediated Phosphorylation Author(s): Dash, Raju; Tran, Non-Nuoc; Lee, Sung Bae; Lee, Byung-Hoon Abstract: Ubiquitin-specific protease 14 (USP14), one of the three major proteasome-associated deubiquitinating enzymes (DUBs), is known to be activated by the AKT-mediated phosphorylation at Ser432. Thereby, AKT can regulate global protein degradation by controlling the ubiquitin-proteasome system (UPS). However, the exact molecular mechanism of USP14 activation by AKT phosphorylation at the atomic level remains unknown. By performing the molecular dynamics (MD) simulation of the USP14 catalytic domain at three different states (inactive, active, and USP14-ubiquitin complex), we characterized the change in structural dynamics by phosphorylation. We observed that the Ser432 phosphorylation induced substantial conformational changes of USP14 in the blocking loop (BL) region to fold it from an open loop into a β-sheet, which is critical for USP14 activation. Furthermore, phosphorylation also increased the frequency of critical hydrogen bonding and salt bridge interactions between USP14 and ubiquitin, which is essential for DUB activity. Structural dynamics insights from this study pinpoint the important local conformational landscape of USP14 by the phosphorylation event, which would be critical for understanding USP14-mediated proteasome regulation and designing future therapeutics. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 2024-05-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/56569 Title: Biallelic USP14 variants cause a syndromic neurodevelopmental disorder Author(s): Ebstein, Frédéric; Latypova, Xenia; Sharon Hung, Ka Ying; Prado, Miguel A.; Lee, Byung-Hoon; Möller, Sophie; Wendlandt, Martin; Zieba, Barbara A.; Florenceau, Laëtitia; Vignard, Virginie; Poirier, Léa; Toutain, Bérénice; Moroni, Isabella; Dubucs, Charlotte; Chassaing, Nicolas; Horvath, Judit; Prokisch, Holger; Küry, Sébastien; Bézieau, Stéphane; Paulo, Joao A.; Finley, Daniel; Krüger, Elke; Ghezzi, Daniele; Isidor, Bertrand Abstract: Purpose: Imbalances in protein homeostasis affect human brain development, with the ubiquitin-proteasome system (UPS) and autophagy playing crucial roles in neurodevelopmental disorders (NDD). This study explores the impact of biallelic USP14 variants on neurodevelopment, focusing on its role as a key hub connecting UPS and autophagy. Methods: Here, we identified biallelic USP14 variants in 4 individuals from 3 unrelated families: 1 fetus, a newborn with a syndromic NDD and 2 siblings affected by a progressive neurological disease. Specifically, the 2 siblings from the latter family carried 2 compound heterozygous variants c.8T>C p.(Leu3Pro) and c.988C>T p.(Arg330∗), whereas the fetus had a homozygous frameshift c.899_902del p.(Lys300Serfs∗24) variant, and the newborn patient harbored a homozygous frameshift c.233_236del p.(Leu78Glnfs∗11) variant. Functional studies were conducted using sodium dodecyl-sulfate polyacrylamide gel electrophoresis, western blotting, and mass spectrometry analyses in both patient-derived and CRISPR-Cas9-generated cells. Results: Our investigations indicated that the USP14 variants correlated with reduced N-terminal methionine excision, along with profound alterations in proteasome, autophagy, and mitophagy activities. Conclusion: Biallelic USP14 variants in NDD patients perturbed protein degradation pathways, potentially contributing to disorder etiology. Altered UPS, autophagy, and mitophagy activities underscore the intricate interplay, elucidating their significance in maintaining proper protein homeostasis during brain development. © 2024 The Authors. Published by Elsevier Inc. on behalf of American College of Medical Genetics and Genomics. This is an open access article under the CC BY license(http://creativecommons.org/licenses/by/4.0/). 2024-05-31T15:00:00Z