https://scholar.dgist.ac.kr/handle/20.500.11750/13657 2026-08-06T16:45:10Z https://scholar.dgist.ac.kr/handle/20.500.11750/60465 Title: Multi-scale imaging and recording of in-vivo neural activity Author(s): Lee, Min Yong; Kim, Hyo Won; Lee, Kwang Abstract: Most in vivo neural technologies have been developed to enhance the resolution of subcellular neuronal activity or to expand the spatial range for tracking ensembles of neurons in the brains of live animals. While these approaches offer great promise for understanding cellular and circuit functions in behaving mammals, their cross-sectional observations are inherently limited in accounting for causal interactions of neural dynamics across various scales of brain architecture. Consequently, the simultaneous observation of multi-scale neural activity has emerged as a crucial strategy for achieving a more comprehensive understanding of brain function. These advancements facilitate the simultaneous detection of diverse signals, providing unprecedented insights into dynamic neurophysiological mechanisms within three-dimensional brain structures that remain poorly understood. Here, we review state-of-the-art technologies for the parallel observation of multiple neural targets in vivo. We highlight strategies for simultaneously observing brain signals at multiple resolutions, aiming to bridge the spatiotemporal gaps between microscopic and macroscopic domains of neurobiology. We also emphasize the technical integration of neural tools to concurrently acquire electrophysiological activity and optical imaging, leveraging their complementary strengths. Finally, we discuss the future challenges and potential prospects of multimodal neural techniques, paving the way for a deeper understanding of brain functions and disorders. [BMB Reports 2026; 59(2): 124-136] 2026-01-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59934 Title: Widefield cortical activity and functional connectivity during motorized locomotion Author(s): Lee, Chang Hak; Lee, Gawon; Song, Hyejin; Lee, Kwang Abstract: The ability to move within a given environment necessitates constant regulation of sensory and motor functions. However, intricacies of sensory-motor integration via intercortical signal correlation remain to be fully elucidated. In this study, we dissociated internally driven cortical dominance from original signals by removing the influence of behavior variables during locomotion on motorized treadmill, wheel, and disk. There were no significant differences in either original or internally driven activity across the cortex of mice during walking based on the type of track. However, the spatial pattern of internally driven cortical connectivity depended on the track type. Especially, internally driven functional connectivity during sustained locomotion on the treadmill significantly decreased only in the medial M2 regions. Thus, the maintenance of stable locomotion on a linear runway is indicative of successful internal sensory-motor integration, which is achieved through inhibitory control of M2. Our findings demonstrate that the spatial patterns of cortical functional connectivity during locomotion are altered by the gait kinematics following physical rotation of the track. Furthermore, we suggest that understanding of health and disorder related to locomotion in environmental contexts requires the consideration of internally driven activity and functional connectivity across the widefield cortex. 2025-12-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59929 Title: Translational reprogramming of dentate gyrus peptidergic circuitry gates antidepressant efficacy Author(s): Oh, Seo-Jin; Jang, Jin-Hyeok; Roussarie, Jean-Pierre; Jang, Kyung-un; Jeong, Min-Seok; Jo, Yeon Suk; Shin, Chang Hun; Choi, Hongsoo; Lee, Kwang; Yoon, Jong-Hyeok; Oh, Yong-Seok Abstract: Selective serotonin reuptake inhibitors (SSRIs) exhibit delayed therapeutic effects despite rapid serotonin elevation, suggesting their dependence on slow neuroplastic adaptations. Here, we demonstrate that antidepressant actions require cell type-specific translational regulation of the peptidergic signaling in the dentate gyrus (DG). Chronic, but not acute, treatment with an SSRI fluoxetine (FLX) selectively enhances translational activity in hilar mossy cells (MCs), with no detectable changes in neighboring granule cells (GCs). Combining Translating Ribosome Affinity Purification (TRAP) with RNA sequencing revealed distinct baseline translatomes between these two glutamatergic neurons and identified FLX-induced remodeling of peptidergic pathways in the DG. Crucially, we discovered MC-specific enrichment of the neuropeptide PACAP, which undergoes translation-dependent upregulation by chronic FLX treatment. This PACAP induction mediates neuroadaptive plasticity in PAC1 receptor-expressing GCs and drives behavioral responses prominently in female mice during prolonged FLX administration. Our findings establish cell type-specific translational reprogramming as a novel mechanistic framework for antidepressant action. 2026-01-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59891 Title: Optical dual brain signal tester Author(s): 김효원; 이민용; 박영권; 이광 Abstract: The present invention relates to an optical fiber photometric measurement technique. An optical brain signal measurement device (100) according to one embodiment may comprise a light source unit (110), an excitation filter unit (120), a first split-splice unit (130), a second split-splice unit (140), an emission filter unit (150), and a measurement unit (160). The optical brain signal measurement device 100 transmits an optical signal to a brain signal measurement object through a directional optical fiber, divides or splices the signal through a dividing and splicing part, and recognizes a wavelength range corresponding to each of the multiple signals through an optical splitter, thereby eliminating a dichroic filter. Therefore, multiple brain signals (multiple light sources) can be obtained by using the light source directivity of the optical fiber and the optical splitter.