https://scholar.dgist.ac.kr/handle/20.500.11750/179 2026-04-06T09:49:07Z https://scholar.dgist.ac.kr/handle/20.500.11750/4976 Title: 세포 자동 계수를 위한 광학현미경 이미지 처리 Author(s): 조미경; 문상준; 심재술 Abstract: 나노 바이오산업의 발전과 더불어 세포 성장 과정에서 발견되는 세포의 이동, 분열, 통합, 아포토시스(apoptosis), 모양 변형, 세포들 간의 상호 작용 등을 포함하는 세포의 행동을 분석하기 위한 자동화된 시스템의 개발은 매우 중요하다. 본 연구에서는 세포 배양 과정에서 광학현미경을 통해 얻은 세포의 실시간 이미지들의 변화/변형 과정을 2D 또는 3D 분석 하기위한 전처리 방법과 세포와 클러스터(둘 이상의 세포의 결합)를 자동 식별하기 위한 방법, 시간의 흐름에 따라 변화되는 세포와 클러스터의 개수를 계수하기 위한 방법을 제시한다. 제안된 방법들은 30분 간격으로 촬영한 3T3 세포 배양 이미지들을 이용하여 실험하였으며 세포 및 클러스터를 분류하고 각각의 개수를 자동 계수한 결과 평균 99.8%의 정확도를 보여 주었다. 2011-10-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/3283 Title: A robust cell counting approach based on a normalized 2D cross-correlation scheme for in-line holographic images Author(s): Ra, Ho Kyeong; Kim, Hyung Seok; Yoon, Hee Jung; Son, Sang Hyuk; Park, Tae Joon; Moon, Sang Jun Abstract: To achieve the important aims of identifying and marking disease progression, cell counting is crucial for various biological and medical procedures, especially in a Point-Of-Care (POC) setting. In contrast to the conventional manual method of counting cells, a software-based approach provides improved reliability, faster speeds, and greater ease of use. We present a novel software-based approach to count in-line holographic cell images using the calculation of a normalized 2D cross-correlation. This enables fast, computationally-efficient pattern matching between a set of cell library images and the test image. Our evaluation results show that the proposed system is capable of quickly counting cells whilst reliably and accurately following human counting capability. Our novel approach is 5760 times faster than manual counting and provides at least 68% improved accuracy compared to other image processing algorithms. © The Royal Society of Chemistry 2013. 2012-12-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/2457 Title: AMP-activated protein kinase: implications on ischemic diseases Author(s): Ahn, Yong-Joo; Kim, Hwewon; Lim, Heejin; Lee, Max; Kang, Yuhyun; Moon, SangJun; Kim, Hyeon Soo; Kim, Hyung-Hwan Abstract: Ischemia is a blockage of blood supply due to an embolism or a hemorrhage in a blood vessel. When an organ cannot receive oxygenated blood and can therefore no longer replenish its blood supply dueto ischemia, stresses, such as the disruption of blood glucose homeostasis, hypoglycemia and hypoxia, activate the AMPK complex. LKB1 and CaMKKβ are essential activators of the AMPK signaling pathway. AMPK triggers proangiogenic effects through the eNOS protein in tissues with ischemic conditions, where cells are vulnerable to apoptosis, autophagy and necrosis. The AMPK complex acts to restore blood glucose levels and ATP levels back to homeostasis. This review will discuss AMPK, as well as its key activators (LKB1 and CaMKKβ), as a central energy regulator and evaluate the upstream and downstream regulating pathways of AMPK. We will also discuss how we can control this important enzyme in ischemic conditions to prevent harmful effects in patients with vascular damage. © 2012 by the The Korean Society for Biochemistry and Molecular Biology. 2012-08-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/2362 Title: Spirally-patterned pinhole arrays for long-term fluorescence cell imaging Author(s): Koo, BU[Koo, Bon Ung]; Kang, Y[Kang, YooNa]; Moon, S[Moon, SangJun]; Lee, WG[Lee, Won Gu] Abstract: Fluorescence cell imaging using a fluorescence microscope is an extensively used technique to examine the cell nucleus, internal structures, and other cellular molecules with fluorescence response time and intensity. However, it is difficult to perform high resolution cell imaging for a long period of time with this technique due to necrosis and apoptosis depending on the type and subcellular location of the damage caused by phototoxicity. A large number of studies have been performed to resolve this problem, but researchers have struggled to meet the challenge between cellular viability and image resolution. In this study, we employ a specially designed disc to reduce cell damage by controlling total fluorescence exposure time without deterioration of the image resolution. This approach has many advantages such as, the apparatus is simple, cost-effective, and easily integrated into the optical pathway through a conventional fluorescence microscope. © 2015 The Royal Society of Chemistry. 2014-12-31T15:00:00Z