https://scholar.dgist.ac.kr/handle/20.500.11750/317 2026-04-04T09:04:36Z https://scholar.dgist.ac.kr/handle/20.500.11750/59909 Title: Erythropoietin-derived Non-erythropoietic Peptides Conferring Oxidative Stress Resistance to Keratinocytes and Fibroblasts Author(s): Han, Min Ae; Ashim, Janbolat; Ji, Youngheum; Kang, Eunho; Jeong, Minchan; Kim, Sung Jae; Yu, Wookyung; Kim, Jin Hae; Moon, Cheil; Lee, Chang-Hun Abstract: Erythropoietin (EPO) exerts tissue-protective effects; however, its erythropoietic activity limits broader use. Three EPO-derived peptides (ML1-C1/C2/C3) were designed from the C-helix of EPO to remove erythropoietic activity while retaining cell-protective activity. Circular dichroism and nuclear magnetic resonance spectroscopies were used to assess the solution structures of ML1-C1/C2/C3 peptides. The peptide activities for cytoprotection and growth support were assessed using skin-relevant cells, HaCaT cells and 3T3-L1 cells, which proposes an effect on skin epithelial keratinocytes and pre-adipocytic fibroblasts, respectively. Also, an erythroid-precursor cell line, TF-1, was used to evaluate the erythropoietic function of the three peptides. Spectroscopic analyses of ML1-C1/C2/C3 peptides revealed similar secondary structures and different flexibilities between the peptides. While ML1-C1 and ML1-C3 had highly flexible loop-like structures, ML1-C2 had less flexible loop-like structures. Also, their cellular effects vary in a cell type-dependent manner. The EPO-derived peptides can attenuate H2O2-induced loss of viability in HaCaT cells and 3T3-L1 cells. Under low-serum conditions, the three peptides promoted HaCaT proliferation, whereas only ML1-C1 improved 3T3-L1 proliferation. In TF-1 cells, none of the peptides increased cell viability or hemoglobin staining, whereas recombinant human EPO did, indicating the lack of erythropoietic activity of the peptides under experimental conditions. These findings support the potential of EPO-derived peptides as skin-protective agents and motivate future work for skin therapeutics or cosmetic purposes. https://scholar.dgist.ac.kr/handle/20.500.11750/59310 Title: 물리화학적 특성과 인지적 특성 데이터베이스를 기반으로 하는 향료 특성 예측 시스템 Author(s): 문제일; 배지섭 https://scholar.dgist.ac.kr/handle/20.500.11750/59222 Title: Stable olfactory receptor activation across odor complexity Author(s): Kim, Minseok; Lee, Jeongyoon; Park, Inah; Kim, Jihoon; Lee, Keunsoon; So, Jinhyun; Choi, Ji-Woong; Jang, Jae Eun; Kwon, Hyuk-Jun; Moon, Cheil; Choe, Han Kyoung Abstract: Mechanisms underlying single odorant activation of specific olfactory receptors are well understood. However, how the olfactory system processes complex odor mixtures at the receptor level remains unclear. This study examined olfactory receptor activation patterns across odor complexities using phosphoTRAP analysis. For most mixtures, receptor activation patterns closely matched the linear sum of individual component responses. However, distinct receptor sets display non-linear responses unexplained by linear models. Mixture responses were generally located between component responses and often aligned with linear predictions, though some deviations indicated non-linear interactions. Total activated receptors remained relatively constant regardless of odor complexity, suggesting efficient coding that prevented receptor saturation as odorant components increased. These findings provide receptor-level evidence that the olfactory system encodes complex odors primarily through linear integration of receptor activity, with added specificity from non-linear responses in limited receptors, advancing understanding of how the olfactory system normalizes receptor activation in response to natural odors. 2025-10-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59082 Title: 물리화학적 특성과 인지적 특성 데이터베이스를 기반으로 하는 향료 특성 예측 시스템 Author(s): 문제일; 배지섭 Abstract: 본 발명은 물리화학적 특성과 인지적 특성 데이터베이스를 기반으로 하는 향료 특성 예측 시스템에 관한 것이다. 본 발명에 따르면, 물리화학적 특성과 인지적 특성 데이터베이스를 기반으로 하는 향료 특성 예측 시스템에 있어서, 복수의 향료 별로 상기 향료에 매칭된 고차원 물리화학적 특성 데이터를 주성분 분석 기법을 통해 분석하여 3차원 물리화학적 특성 좌표계 상의 3차원 데이터로 투영하는 데이터 변환부와, 상기 향료 별 기 매칭된 인지적 특성 카테고리 정보를 바탕으로, 상기 3차원 물리화학적 특성 좌표계 내의 각 향료 별 3차원 좌표를 상기 인지적 특성 카테고리 별로 그룹핑하고, 그룹핑된 각 인지적 특성 카테고리 별 중심점 좌표를 연산하는 그룹핑부, 및 미지 향료에 대한 물리화학적 특성 벡터를 입력받아 상기 3차원 물리화학적 좌표계 상의 3차원 좌표로 변환 후 상기 변환한 3차원 좌표와 상기 인지적 특성 카테고리 별 중심점 간 거리를 토대로 미지 향료의 인지적 특성 카테고리를 예측하는 제어부를 포함한다. 본 발명에 따르면, 새로운 향료에 대한 물리화학적 특성을 예측기에 입력하여 해당 향료에 대해 예상되는 인지적 특성을 용이하게 도출해낼 수 있다.