https://scholar.dgist.ac.kr/handle/20.500.11750/319 2026-04-04T10:54:24Z https://scholar.dgist.ac.kr/handle/20.500.11750/59909 Title: Erythropoietin-derived Non-erythropoietic Peptides Conferring Oxidative Stress Resistance to Keratinocytes and Fibroblasts Author(s): Han, Min Ae; Ashim, Janbolat; Ji, Youngheum; Kang, Eunho; Jeong, Minchan; Kim, Sung Jae; Yu, Wookyung; Kim, Jin Hae; Moon, Cheil; Lee, Chang-Hun Abstract: Erythropoietin (EPO) exerts tissue-protective effects; however, its erythropoietic activity limits broader use. Three EPO-derived peptides (ML1-C1/C2/C3) were designed from the C-helix of EPO to remove erythropoietic activity while retaining cell-protective activity. Circular dichroism and nuclear magnetic resonance spectroscopies were used to assess the solution structures of ML1-C1/C2/C3 peptides. The peptide activities for cytoprotection and growth support were assessed using skin-relevant cells, HaCaT cells and 3T3-L1 cells, which proposes an effect on skin epithelial keratinocytes and pre-adipocytic fibroblasts, respectively. Also, an erythroid-precursor cell line, TF-1, was used to evaluate the erythropoietic function of the three peptides. Spectroscopic analyses of ML1-C1/C2/C3 peptides revealed similar secondary structures and different flexibilities between the peptides. While ML1-C1 and ML1-C3 had highly flexible loop-like structures, ML1-C2 had less flexible loop-like structures. Also, their cellular effects vary in a cell type-dependent manner. The EPO-derived peptides can attenuate H2O2-induced loss of viability in HaCaT cells and 3T3-L1 cells. Under low-serum conditions, the three peptides promoted HaCaT proliferation, whereas only ML1-C1 improved 3T3-L1 proliferation. In TF-1 cells, none of the peptides increased cell viability or hemoglobin staining, whereas recombinant human EPO did, indicating the lack of erythropoietic activity of the peptides under experimental conditions. These findings support the potential of EPO-derived peptides as skin-protective agents and motivate future work for skin therapeutics or cosmetic purposes. https://scholar.dgist.ac.kr/handle/20.500.11750/59222 Title: Stable olfactory receptor activation across odor complexity Author(s): Kim, Minseok; Lee, Jeongyoon; Park, Inah; Kim, Jihoon; Lee, Keunsoon; So, Jinhyun; Choi, Ji-Woong; Jang, Jae Eun; Kwon, Hyuk-Jun; Moon, Cheil; Choe, Han Kyoung Abstract: Mechanisms underlying single odorant activation of specific olfactory receptors are well understood. However, how the olfactory system processes complex odor mixtures at the receptor level remains unclear. This study examined olfactory receptor activation patterns across odor complexities using phosphoTRAP analysis. For most mixtures, receptor activation patterns closely matched the linear sum of individual component responses. However, distinct receptor sets display non-linear responses unexplained by linear models. Mixture responses were generally located between component responses and often aligned with linear predictions, though some deviations indicated non-linear interactions. Total activated receptors remained relatively constant regardless of odor complexity, suggesting efficient coding that prevented receptor saturation as odorant components increased. These findings provide receptor-level evidence that the olfactory system encodes complex odors primarily through linear integration of receptor activity, with added specificity from non-linear responses in limited receptors, advancing understanding of how the olfactory system normalizes receptor activation in response to natural odors. 2025-10-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/58956 Title: Nasal Aβ42 mirrors brain amyloid dynamics and cognitive decline across the Alzheimer’s disease continuum Author(s): Jung, Da Hae; Son, Gowoon; Wang, Sheng Min; Yoo, Seung-Jun; Jahanshahi, Ali; Lim, Hyun Kook; Moon, Cheil Abstract: Early, non-invasive assessment of Alzheimer’s disease (AD) progression remains a key challenge. This study evaluated whether nasal amyloid-β42 (Aβ42) levels reflect brain amyloid dynamics and cognitive decline. Nasal discharge from 161 individuals, ranging from cognitively unimpaired to AD dementia, was analyzed using ELISA, alongside neuropsychological assessments and amyloid PET imaging. Moderate nasal Aβ42 levels (9.53–11.10pg/mL) were positively associated with PET amyloid burden and cognitive decline, identifying a critical transitional disease stage. Conversely, the highest Aβ42 levels showed weaker correlations, suggesting a non-linear progression. The pattern of nasal Aβ42 mirrored brain amyloid accumulation, which peaks and stabilizes in later disease stages. These findings highlight nasal Aβ42 as a promising, scalable biomarker for tracking AD pathology and offer the first evidence linking it with brain amyloid PET. This supports its potential use in both clinical and longitudinal research settings. © 2025 Elsevier B.V., All rights reserved. 2025-07-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/58386 Title: 표피에 발현하는 이소성 후각수용체를 활용한 신개념 화장품 후보 활성물질 선별 시스템 개발 Author(s): 류상은; 심다미; 문제일 2025-04-30T15:00:00Z